Attogram per milliliter detection of prostate cancer biomarker using polymer dots-based electrolyte-gated organic field-effect transistor

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2026-06-01 Epub Date: 2026-02-02 DOI:10.1016/j.bios.2026.118476

Samira Mansouri Majd , Fatemeh Mirzapour , Mojtaba Shamsipur , Ziba Nematifar

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Abstract

Early detection of biomarkers in body fluids plays a critical role in the diagnosis and prognosis of prostate cancer, the second most common cancer in men. In this study, we synthesized novel semiconducting polymer dots (Pdots) to serve as the channel material in electrolyte-gated organic field-effect transistors (EGOFET) for the ultrasensitive detection of PSA antigen, a key cancer biomarker. To fabricate the active channel material, Pdots were drop-cast onto the FET surface, and the surface was subsequently functionalized with PSA antibodies. The Pdot EGOFET immunosensor demonstrated the ability to detect PSA antigen across a concentration range of 10 ag/mL to 10 ng/mL, with a significant decrease in drain current (I_ds) and a high sensitivity of 0.9097 μA/decade. The sensor exhibited a limit of detection (LOD) of 3.0 ag/mL. Furthermore, the Pdot EGOFET immunosensor displayed remarkable electrical characteristics, including a mobility of 0.029 cm²V⁻¹s⁻¹, an on/off current ratio of 22.66, and a subthreshold swing (SS) of 909.1 mV/decade. Additionally, the sensor demonstrated excellent selectivity, with its performance being compared favorably to an enzyme-linked immunosorbent assay (ELISA) for detecting PSA in spiked human serum samples. The positive and promising results of this study indicate that the developed Pdot EGOFET immunosensor holds significant potential as a highly effective platform for cancer diagnosis and therapeutic monitoring.

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基于聚合物点电解质门控有机场效应晶体管的前列腺癌生物标志物每毫升图检测

前列腺癌是男性第二大常见癌症，体液中生物标志物的早期检测在前列腺癌的诊断和预后中起着至关重要的作用。在这项研究中，我们合成了一种新型的半导体聚合物点（Pdots）作为电解门控有机场效应晶体管（EGOFET）的通道材料，用于PSA抗原的超灵敏检测，PSA抗原是一种关键的癌症生物标志物。为了制造活性通道材料，将Pdots滴铸到FET表面，然后用PSA抗体对表面进行功能化。Pdot EGOFET免疫传感器能够检测浓度范围为10 ag/mL至10 ng/mL的PSA抗原，漏极电流（Ids）显著降低，灵敏度高达0.9097 μA/decade。该传感器的检出限为3.0 ag/mL。此外，Pdot EGOFET免疫传感器具有显著的电特性，包括迁移率为0.029 cm2V−1s−1，通/关电流比为22.66，亚阈值摆幅（SS）为909.1 mV/ 10年。此外，该传感器表现出优异的选择性，其性能优于酶联免疫吸附试验（ELISA），用于检测加标的人血清样品中的PSA。本研究的积极和有希望的结果表明，所开发的Pdot EGOFET免疫传感器具有作为癌症诊断和治疗监测的高效平台的巨大潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.