Selenium modulates the antioxidative response in green microalga Monoraphidium cf. contortum

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2026-04-01 Epub Date: 2026-02-09 DOI:10.1016/j.etap.2026.104958

Dubravka Špoljarić Maronić , Ivna Štolfa Čamagajevac , Tanja Žuna Pfeiffer , Ana Pilipović , Nikolina Sabo , Nikolina Bek , Filip Stević , Ivana Maksimović , Anita Galir , Zdenko Lončarić

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Abstract

As algae are the main route of selenium (Se) transport from water to other consumers in food webs, the effect of selenite on the total Se content, growth, and antioxidant status of the unicellular green alga Monoraphidium cf. contortum was determined. The basal Se content (<3 mg/kg DW) was lower compared to other green microalgae, but the species showed a high accumulation potential (284.1 mg/kg DW). Despite early Se accumulation and growth inhibition, a significant loss of chlorophylls and carotenoids was observed only after 72 h. The response of antioxidative enzymes corresponded to a lower intensity of lipid peroxidation, despite increased ROS content at lower selenite concentrations. Primarily, selenite increased the activities of ascorbate peroxidase (APX), glutathione reductase, glutathione peroxidase (GPX), and glutathione S-transferase (GST), and inhibited catalase (CAT) in almost all treatments. Higher GST and GPX activities, as well as selenite-induced non-enzymatic glutathione (GSH) oxidation indicated increased GSH depletion.

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硒调节绿微藻的抗氧化反应

由于藻类是食物网中硒从水中转运到其他消费者的主要途径，因此研究了亚硒酸盐对单细胞绿藻（Monoraphidium cf. contortum）总硒含量、生长和抗氧化状态的影响。其硒的基础含量（3mg/kg DW）低于其他绿微藻，但积累潜力较大（284.1mg/kg DW）。尽管早期硒积累和生长抑制，但仅在72小时后才观察到叶绿素和类胡萝卜素的显著损失。尽管亚硒酸盐浓度较低时ROS含量增加，但抗氧化酶的反应与较低强度的脂质过氧化反应相对应。在几乎所有处理中，亚硒酸盐增加了抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶、谷胱甘肽过氧化物酶（GPX）和谷胱甘肽s -转移酶（GST）的活性，抑制了过氧化氢酶（CAT）的活性。较高的GST和GPX活性，以及亚硒酸盐诱导的非酶促谷胱甘肽（GSH）氧化表明GSH消耗增加。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.