Photoacoustic imaging and biodistribution analysis of trimethyl chitosan-stabilized ZnO nanoparticles embedded in nanofiber scaffolds for diabetic wound healing

IF 4.6 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2026-03-01 Epub Date: 2026-02-03 DOI:10.1016/j.nano.2026.102908

Ankit Kumar Malik , Pooja Goswami , Vaishali , Rajesh Saini , Datta Maroti Pawde , Aseem Setia , Biplob Koch , Madaswamy S. Muthu

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Abstract

Chronic wounds associated with diabetes represent a persistent clinical challenge, primarily due to their delayed healing dynamics and heightened vulnerability to microbial invasion, which can precipitate serious adverse outcomes. In response to these issues, we have developed a nanofiber scaffold loaded with trimethyl chitosan-derived ZnO nanoparticles (ZnO-TMC-NPs-NFs) to enhance diabetic wound-healing therapy. The developed nanoparticle (ZnO-TMC-NPs) has a size of 16.1 ± 3.7 nm and a zeta potential of +26.3 ± 1.7 mV. Integration of ZnO-TMC-NPs into chitosan poly(vinyl-alcohol) nanofiber scaffolds was performed to develop nanoformulation ranging between 120 and 240 nm. Moreover, the robustness of this research is enhanced by in vitro cell line evaluations using L929 mouse fibroblast and A549 lung cancer cell lines, and by integrating in vivo optical modalities with advanced ultrasound/photoacoustic (PA) imaging. Collectively, the results underscore the therapeutic promise of this nanofibrous scaffold, particularly when functionalized with TMC-ZnO, as a viable intervention for diabetic wound management.

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纳米纤维支架中三甲基壳聚糖稳定ZnO纳米粒子的光声成像和生物分布分析

糖尿病相关的慢性伤口是一个持续的临床挑战，主要是因为它们的愈合动力学延迟和对微生物入侵的脆弱性增加，这可能导致严重的不良后果。针对这些问题，我们开发了一种负载三甲基壳聚糖衍生ZnO纳米颗粒（ZnO- tmc - nps - nfs）的纳米纤维支架，以增强糖尿病伤口愈合治疗。纳米粒子（ZnO-TMC-NPs）粒径为16.1 ± 3.7 nm， zeta电位为+26.3 ± 1.7 mV。将ZnO-TMC-NPs整合到壳聚糖聚乙烯醇纳米纤维支架中，得到了120 ~ 240 nm的纳米配方。此外，通过使用L-929小鼠成纤维细胞和A-549肺癌细胞系进行体外细胞系评估，以及将体内光学模式与先进的超声/光声（PA）成像相结合，增强了本研究的稳健性。总的来说，这些结果强调了这种纳米纤维支架的治疗前景，特别是当与TMC-ZnO功能化时，作为糖尿病伤口管理的可行干预措施。

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来源期刊

Nanomedicine : nanotechnology, biology, and medicine 工程技术-纳米科技

CiteScore

11.10

自引率

0.00%

发文量

133

审稿时长

42 days

期刊介绍： The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.