Salivary monoacetylated polyamines as noninvasive biomarkers for early detection and stratification of oral squamous cell carcinoma: A targeted metabolomics study

IF 2.3 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology Pub Date : 2026-04-01 Epub Date: 2026-01-09 DOI:10.1016/j.archoralbio.2026.106513

Karthika Panneerselvam , Rajkumar Krishnan , Sathishkumar Mahadevan , Mathan Mohan Ayyadurai , Masahiro Sugimoto

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引用次数: 0

Abstract

Objectives

This study aimed to evaluate salivary monoacetylated polyamines as noninvasive biomarkers for the detection and staging of oral squamous cell carcinoma (OSCC).

Design

Unstimulated saliva samples were collected from healthy controls (n = 15), oral leukoplakia (OLK) patients (n = 15), and OSCC patients stratified into early (n = 28) and advanced stages (n = 46). Seven polyamines were quantified using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Diagnostic performance was assessed via receiver operating characteristic (ROC) analysis, logistic regression modeling, and multivariate clustering.

Results

N¹-acetylspermidine and N¹-acetylspermine showed progressive elevation from OLK to advanced OSCC, with AUCs of 0.87 and 0.81, respectively. A two-marker logistic model achieved a cross-validated AUC of 0.85, demonstrating good discriminatory performance between malignant and non-malignant states. Multivariate analysis confirmed their contribution to disease stratification, while calibration plots supported model reliability.

Conclusions

Salivary N¹-acetylspermidine and N¹-acetylspermine are promising biomarkers for noninvasive OSCC detection and staging.

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唾液单乙酰化多胺作为口腔鳞状细胞癌早期检测和分层的无创生物标志物：一项靶向代谢组学研究

目的：本研究旨在评估唾液单乙酰化多胺作为口腔鳞状细胞癌（OSCC）检测和分期的无创生物标志物。设计：收集健康对照（n = 15）、口腔白斑（n = 15）患者（n = 15）和早期（n = 28）和晚期（n = 46）OSCC患者的未刺激唾液样本。采用液相色谱-串联质谱法（LC-MS/MS）对7种多胺进行定量分析。通过受试者工作特征（ROC）分析、逻辑回归模型和多变量聚类来评估诊断效果。结果：n1 -乙酰精胺和n1 -乙酰精胺从OLK到晚期OSCC呈进行性升高，auc分别为0.87和0.81。双标记逻辑模型的交叉验证AUC为0.85，表明恶性和非恶性状态之间具有良好的区分性能。多变量分析证实了它们对疾病分层的贡献，而校准图支持模型的可靠性。结论：唾液n1 -乙酰精胺和n1 -乙酰精胺是有希望用于无创OSCC检测和分期的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of oral biology 医学-牙科与口腔外科

CiteScore

5.10

自引率

3.30%

发文量

177

审稿时长

26 days

期刊介绍： Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry