Rapid identification of chemical components in Polygonatum kingianum Collett & Hemsl. based on UHPLC-Q-Exactive orbitrap MS coupled with molecular networking

IF 2.5 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B Pub Date : 2026-03-15 Epub Date: 2026-01-31 DOI:10.1016/j.jchromb.2026.124943

Qing Li , Kailin Li , Jiatong Yang , Wen Cai , Huiqing Yang , Bowen Gao , Wei Cai

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Abstract

Polygonatum kingianum Collett & Hemsl. (PK) is a plant dually used as both medicine and food, renowned in traditional medicine for its abilities to replenish qi, nourish yin, moisten the lungs, and benefit the kidneys. Previous investigations have indicated that its chemical composition encompasses steroidal saponins, polysaccharides, and flavonoids. However, the PK's chemical components is still scarce. Therefore, it is essential to establish a systematic method for the identifying and characterizing its chemical components. In this study, a total of 153 chemical components, including 37 peptides, 26 organic acids, 20 saponins, 19 flavonoids, 7 amino acids, 7 nucleotides, 7 lipids, 6 terpenoids and other classes, was identified by UHPLC-Q-Exactive Orbitrap MS with molecular networking. This analysis revealed 118 new compounds from PK, which distinctly lays the foundation for the scientific exploration of the pharmacodynamic substance basis of PK as well as the development and application of the resources derived from it.

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黄精化学成分的快速鉴定。基于UHPLC-Q-Exactive轨道阱质谱联用分子网络。

黄精（PK）是一种药食两用的植物，在传统医学中以其补气、养阴、润肺、益肾而闻名。先前的研究表明，其化学成分包括甾体皂苷、多糖和类黄酮。然而，PK的化学成分仍然稀缺。因此，有必要建立一种系统的方法来鉴定和表征其化学成分。本研究利用UHPLC-Q-Exactive Orbitrap MS结合分子网络，共鉴定了153种化学成分，包括37种多肽、26种有机酸、20种皂苷、19种黄酮类、7种氨基酸、7种核苷酸、7种脂质、6种萜类等。通过分析，从PK中发现了118个新化合物，为科学探索PK的药效学物质基础和开发利用PK衍生资源奠定了坚实的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.