Characterisation of pyridaben resistance in Tetranychus urticae in the Mediterranean basin

IF 3.3 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2026-04-01 Epub Date: 2026-01-30 DOI:10.1016/j.cropro.2026.107552

Animesha Rath , Preetom Regon , Svetlana Konzedalov, Adi Kliot

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引用次数: 0

Abstract

Tetranychus urticae, the Two-Spotted Spider Mite (TSSM) is a plant pest causing serious economic damage in various crops worldwide. The main means of controlling TSSM in the field are based on chemical pesticides but cases of reported resistance are numerous. Pyridaben is one of the pesticides used against TSSM, however reports from growers in the area indicated that it is losing its efficiency. In this study, we performed a survey testing field populations for their susceptibility to pyridaben over the years 2023–2025. We then randomly selected five populations of TSSM from this survey originating from different geographic locations and with variable resistance abilities to pyridaben. We tested these populations both for cytochrome P450-based metabolic breakdown of pyridaben and for target-site mutations. Our survey showed that pyridaben resistance is highly common in the field with 32 populations of 37 tested being resistant to a dose of pyridaben which is double the recommended dose for commercial use in the field. Furthermore, we show that resistance in most populations is likely to be metabolic, and we use a P450-inhibitor to successfully re-introduce susceptibility to three of four resistance populations. Our data sheds light on the current situation of TSSM resistance to pyridaben in the Mediterranean basin and strongly supports reduction in use of this compound in the near future.

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地中海盆地荨麻疹叶螨抗药性特征分析

荨麻疹叶螨（Tetranychus monticae, Two-Spotted Spider Mite， TSSM）是一种严重危害全球农作物经济的植物害虫。在田间控制TSSM的主要手段是基于化学农药，但报告的抗性病例很多。嘧达虫是用来对付TSSM的一种杀虫剂，然而来自该地区种植者的报告表明，它正在失去效力。在本研究中，我们对2023-2025年的田间种群进行了嘧螨虫易感性的调查测试。然后，我们从这次调查中随机选择了5个来自不同地理位置、对嘧螨抗性不同的TSSM种群。我们对这些人群进行了基于细胞色素p450的pyridaben代谢分解和靶位点突变测试。我们的调查显示，对嘧螨虫抗药性在田间非常普遍，37个试验种群中有32个种群对某剂量的嘧螨虫产生抗药性，该剂量是田间商业使用推荐剂量的两倍。此外，我们发现大多数种群的抗性可能是代谢性的，并且我们使用p450抑制剂成功地重新引入了对四个抗性种群中的三个的敏感性。我们的数据揭示了地中海盆地TSSM对嘧螨虫抗药性的现状，并强烈支持在不久的将来减少该化合物的使用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.