The protective effect of puerarin on the rumen epithelial barrier in heat-stressed beef cattle

IF 3.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2026-03-15 Epub Date: 2026-01-24 DOI:10.1016/j.anifeedsci.2026.116668

Wanwan Wang, Yutong Liu, Xiaozhen Song, Mingren Qu, Yanjiao Li

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Abstract

This study aimed to assess the impacts of puerarin on the growth performance, rumen epithelial morphology, microbiota, and metabolites of beef cattle subjected to heat stress. Thirty-six male simmental hybrid beef cattle (initial weight 606.86 ± 4.73 kg) were randomly allocated into a control diet or a diet containing 400 mg/kg puerarin (dry matter basis) for a 60-days summer trial after a 10-days adaptation period (n = 6, average daily THI = 81.56). The results revealed that puerarin significantly improved (P < 0.05) the average daily gain, dry matter intake, papillae length, thickness of stratum granulosum, stratum spinosum and basale, and total epithelia, interleukin-4 level, contents of microbial crude protein and isovalerate. Puerarin significantly decreased (P < 0.05) thickness of stratum corneum, levels of tumor necrosis factor-α, interleukin-1β, interleukin-6, creatine kinase, lactate dehydrogenase, cortisol, and adreno cortico tropic hormone. Furthermore, puerarin significantly increased (P < 0.05) the abundance of Oribacterium, Streptococcus, Enterococcus and Akkermansia and reduced (P < 0.05) the abundance of Fusobacteriota. Metabolomics analysis revealed that puerarin upregulates the differentially expressed metabolites including O-acetyl- L-serine, pyridoxal phosphate, L-glutathione, reduced, L-alanine, L-aspartic acid, N-acetyl-L-glutamate, pantothenic acid, dodecanoic acid, pyridoxamine, DL-homocysteine, and alpha-ketoglutarate (VIP ≥ 1, t-test P < 0.05). The correlation analysis revealed that Akkermansia exhibited negative correlations with interleukin-1β, interleukin-6, creatine kinase, lactate dehydrogenase, and cortisol (|R| > 0.5, P < 0.05). And Enterococcus exhibited a positive correlation with O-acetyl- L-serine, DL-homocysteine, pyridoxal phosphate, pyridoxamine, and L-glutathione, reduced (R| > 0.5, P < 0.05). In conclusion, 400 mg/kg puerarin supplementation alleviates heat stress by repairing rumen epithelium, optimizing microbial community, and enhancing metabolic functions, thereby improving growth performance of beef cattle.

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葛根素对热应激肉牛瘤胃上皮屏障的保护作用

本研究旨在评估葛根素对热应激肉牛生长性能、瘤胃上皮形态、微生物群和代谢物的影响。选取36头初始体重为606.86 ± 4.73 kg的雄性杂交肉牛，经过10 d的预试期，随机分为对照饲粮和添加400 mg/kg葛根素（干物质基础）的饲粮（n = 6，平均日THI = 81.56），进行60 d的夏季试验。结果表明，葛根素显著提高了仔猪平均日增重、干物质采食量、乳突长度、颗粒层、棘层和基底层厚度以及总上皮、白细胞介素-4水平、微生物粗蛋白和异戊酸含量（P <； 0.05）。葛根素显著降低角质层厚度（P <； 0.05），降低肿瘤坏死因子-α、白细胞介素-1β、白细胞介素-6、肌酸激酶、乳酸脱氢酶、皮质醇、促肾上腺皮质激素水平（P <； 0.05）。葛根素显著提高了Oribacterium、Streptococcus、Enterococcus和Akkermansia的丰度（P <； 0.05），显著降低了Fusobacteriota的丰度（P <； 0.05）。代谢组学分析显示，葛根素上调了o -乙酰- l-丝氨酸、磷酸吡哆醛、l-谷胱甘肽、还原性、l-丙氨酸、l-天冬氨酸、n -乙酰- l-谷氨酸、泛酸、十二烷酸、吡哆胺、dl -同型半胱氨酸和α -酮戊二酸等差异表达的代谢物（VIP≥1，t检验P <； 0.05）。相关性分析显示，Akkermansia与白细胞介素-1β、白细胞介素-6、肌酸激酶、乳酸脱氢酶、皮质醇呈负相关（|R| > 0.5, P <； 0.05）。肠球菌与o -乙酰- l-丝氨酸、dl -同型半胱氨酸、磷酸吡哆醛、吡哆胺、l-谷胱甘肽含量降低呈正相关（R| > 0.5, P <； 0.05）。综上所述，饲粮添加400 mg/kg葛根素可通过修复瘤胃上皮、优化微生物群落、增强代谢功能等方式缓解热应激，从而提高肉牛的生长性能。

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来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.