Synergistic effects of polystyrene microplastics and 17α-methyltestosterone on immune and oxidative stress responses in the gill and liver of Gobiocypris rarus

IF 4.2 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2026-04-01 Epub Date: 2026-01-22 DOI:10.1016/j.fsi.2026.111143

Yibo Jia , Lu Cao , Tongyao Li , Haiyan Zhao , Gen Chen , Zijun Xiong , Weiya Rong , Qing Liu , Weiwei Wang , Jing Song , Xianzong Wang , Yu Liu , Shao-Zhen Liu

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Abstract

This study investigated the individual and combined effects of Polystyrene microplastics (PS) and 17α-methyltestosterone (MT) on the gill and liver of Gobiocypris rarus.Exposure to PS (0.5 mg/L) and MT (50 ng/L) induced significant histopathological alterations and immunotoxicity. Tissues exhibited inflammatory infiltration, nuclear dissolution, and cytoplasmic vacuolation, with the most severe lesions observed under combined exposure. Gene expression analysis revealed significant upregulation of immune and oxidative stress-related genes, including caspase 6 (CASP6), interleukin-1 receptor type I (IL-1RI), NADPH oxidase 1 (NOX1), Toll-like receptor 2 (TLR-2), and C-C motif chemokine receptor 7 (CCR7), while antioxidant enzyme activities and malondialdehyde (MDA) levels were disrupted, indicating enhanced oxidative stress. Transcriptomic analysis of gills further revealed enrichment of ECM-receptor interaction, cell adhesion molecules, and leukocyte transendothelial migration pathways, suggesting that PS and MT may compromise immune function by interfering with extracellular matrix-related signaling, thereby exacerbating tissue damage and posing combined ecological risks in aquatic organisms.

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聚苯乙烯微塑料和17α-甲基睾酮对鱼鳃和肝脏免疫和氧化应激反应的协同作用

本实验研究了聚苯乙烯微塑料（PS）和17α-甲基睾酮（MT）单独或联合对稀松虾鳃和肝脏的影响。暴露于PS （0.5 mg/L）和MT （50 ng/L）诱导了显著的组织病理学改变和免疫毒性。组织表现出炎症浸润、核溶解和细胞质空泡化，在联合暴露下观察到最严重的病变。基因表达分析显示，免疫和氧化应激相关基因，包括半胱天冬酶6 （CASP6）、白细胞介素-1受体I型（IL-1RI）、NADPH氧化酶1 （NOX1）、toll样受体2 （TLR-2）和C-C基序趋化因子受体7 （CCR7）显著上调，而抗氧化酶活性和丙二醛（MDA）水平被破坏，表明氧化应激增强。对鳃的转录组学分析进一步揭示了ecm受体相互作用、细胞粘附分子和白细胞跨内皮迁移途径的富集，表明PS和MT可能通过干扰细胞外基质相关信号而损害免疫功能，从而加剧组织损伤并构成水生生物的综合生态风险。

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.