Bile acid alterations in biliary obstruction patients with clonorchiasis

IF 2.6 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2026-02-01 Epub Date: 2026-01-21 DOI:10.1016/j.actatropica.2026.107989

Shiwen Hua , Xiang Li , Zhenli Xu , Rui Chen , Qinghua Wu , Wenge Yang , Yun Tao , Shanshan Duan , Jian Ding , Jie Wan , Jingjie Lei , Yang Cheng , Yifan Sun , Youyi Liu , Su Han

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引用次数: 0

Abstract

Background

Clonorchiasis is a significant foodborne zoonotic parasitic disease that leads to severe complications such as cholecystitis, liver cirrhosis, and biliary obstruction (BO). Abnormalities in bile acids (BAs) play a crucial role in BO progression. Nevertheless, the abnormalities in BAs and their role in BO patients with Clonorchis sinensis (C. sinensis) infection remain inadequately understood.

Methods

This study included BO patients with or without C. sinensis infection. Bile samples were collected via Endoscopic Retrograde Cholangiopancreatography (ERCP) and bile acid (BA) profiles were analyzed by liquid chromatography electrospray ionization tandem mass spectrometry (LC-MS/MS). Biliary microbiota was analyzed through high-throughput 16S ribosomal RNA (16S rRNA) gene sequencing. Spearman correlation assessed associations between BA, biochemical markers, and biliary microbiota.

Result

Metabolomic analysis revealed that the levels of chenodeoxycholic acid (CDCA), ursodeoxycholic acid (UDCA), hyodeoxycholic acid (HDCA), and 3β-cholic acid (βCA) were significantly reduced in C. s-infected group and positively correlated with Enterococcus abundance. Furthermore, the levels of alkaline phosphatase (ALP) demonstrated a strong positive correlation with CA (cholic acid)/CDCA (r = 0.74, P = 0.01) and (CA+deoxycholic acid [DCA])/(CDCA+lithocholic acid [LCA]) (r = 0.75, P = 0.01) in the C. s-infected group.

Conclusions

BO patients infected with C. sinensis displayed BA profiles, with reduced CDCA, UDCA, HDCA, and β-CA levels correlating with Enterococcus abundance. These preliminary findings suggest an interaction among BA metabolism, the biliary microbiota, and liver damage associated with parasitic infection.

Abstract Image

查看原文本刊更多论文

支睾吸虫病患者胆道梗阻的胆汁酸变化。

背景：支睾吸虫病是一种重要的食源性人畜共患寄生虫病，可导致胆囊炎、肝硬化和胆道梗阻（BO）等严重并发症。胆汁酸（BAs）异常在BO进展中起着至关重要的作用。然而，BAs异常及其在BO患者感染华支睾吸虫（C. sinensis）中的作用仍未充分了解。方法：本研究纳入了伴有或未伴有中华梭菌感染的BO患者。采用内窥镜逆行胆管胰胆管造影（ERCP）采集胆汁样本，采用液相色谱-电喷雾电离串联质谱（LC-MS/MS）分析胆汁酸（BA）谱。通过高通量16S核糖体RNA （16S rRNA）基因测序分析胆道微生物群。Spearman相关性评估BA、生化标记物和胆道微生物群之间的关联。结果：代谢组学分析显示c - s感染组鹅去氧胆酸（CDCA）、熊去氧胆酸（UDCA）、羟基去氧胆酸（HDCA）和3β-胆酸（βCA）水平显著降低，并与肠球菌丰度呈正相关。此外，c - s感染组碱性磷酸酶（ALP）水平与CA（胆酸）/CDCA （r = 0.74,P = 0.01）和（CA+脱氧胆酸[DCA]）/(CDCA+石胆酸[LCA]) （r = 0.75,P = 0.01）呈极显著正相关。结论：感染中华梭菌的BO患者表现出BA谱，CDCA、UDCA、HDCA和β-CA水平的降低与肠球菌丰度相关。这些初步发现表明BA代谢、胆道微生物群和与寄生虫感染相关的肝损伤之间存在相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.