Interaction of ML1 and SRB1-12 mediates cholesterol transport in the brown planthopper

IF 4.3 2区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Insect Biochemistry and Molecular Biology Pub Date : 2026-03-01 Epub Date: 2026-01-16 DOI:10.1016/j.ibmb.2026.104499

Xu Cheng , Yaxin Liu , Chenxu Zeng , Chang Liu , Zihan Zhang , Yanyuan Bao

{"title":"Interaction of ML1 and SRB1-12 mediates cholesterol transport in the brown planthopper","authors":"Xu Cheng , Yaxin Liu , Chenxu Zeng , Chang Liu , Zihan Zhang , Yanyuan Bao","doi":"10.1016/j.ibmb.2026.104499","DOIUrl":null,"url":null,"abstract":"<div><div>Cholesterol transport plays a pivotal role in maintaining sterol homeostasis within eukaryotic cells. In mammals, Niemann-Pick Type C (NPC) proteins are known to participate in cholesterol transport. However, the precise pathways remain largely unexplored in insects. Here, we reported ML1, a lipid-recognition protein in a rice pest <em>Nilaparvata lugens</em>, specifically bound cholesterol. In addition, we identified a scavenger receptor class B Type Ⅰ (SRB1-12) protein as the receptor for ML1. <em>SRB1-12</em> was predominantly expressed in the integument and regulated cuticular chitin biosynthesis and lipid metabolism in nymphs to ensure molting success. ML1 delivered cholesterol to SRB1-12 and supplied the steroid precursor for 20E biosynthesis, as reflected by altered whole-body 20E titers. <em>ML1</em> and <em>SRB1-12</em> co-regulated the expression of a newly identified gene <em>MLSR</em>, which played important roles in nymphal development and egg hatching. This study establishes a pathway of cholesterol trafficking within tissues and deepens the understanding of sterol homeostasis in insects.</div></div>","PeriodicalId":330,"journal":{"name":"Insect Biochemistry and Molecular Biology","volume":"188 ","pages":"Article 104499"},"PeriodicalIF":4.3000,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Insect Biochemistry and Molecular Biology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0965174826000238","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/1/16 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Cholesterol transport plays a pivotal role in maintaining sterol homeostasis within eukaryotic cells. In mammals, Niemann-Pick Type C (NPC) proteins are known to participate in cholesterol transport. However, the precise pathways remain largely unexplored in insects. Here, we reported ML1, a lipid-recognition protein in a rice pest Nilaparvata lugens, specifically bound cholesterol. In addition, we identified a scavenger receptor class B Type Ⅰ (SRB1-12) protein as the receptor for ML1. SRB1-12 was predominantly expressed in the integument and regulated cuticular chitin biosynthesis and lipid metabolism in nymphs to ensure molting success. ML1 delivered cholesterol to SRB1-12 and supplied the steroid precursor for 20E biosynthesis, as reflected by altered whole-body 20E titers. ML1 and SRB1-12 co-regulated the expression of a newly identified gene MLSR, which played important roles in nymphal development and egg hatching. This study establishes a pathway of cholesterol trafficking within tissues and deepens the understanding of sterol homeostasis in insects.

Abstract Image

查看原文本刊更多论文

ML1和SRB1-12的相互作用介导了褐飞虱体内胆固醇的转运。

胆固醇转运在维持真核细胞内固醇稳态中起着关键作用。在哺乳动物中，尼曼-匹克C型（NPC）蛋白参与胆固醇运输。然而，昆虫的确切途径在很大程度上仍未被探索。在这里，我们报道了水稻害虫Nilaparvata lugens中的一种脂质识别蛋白ML1，它可以特异性地结合胆固醇。此外，我们还鉴定出一种清道夫受体B类Ⅰ（SRB1-12）蛋白作为ML1的受体。SRB1-12主要表达于被膜中，调控若虫表皮几丁质的生物合成和脂质代谢，保证了若虫的蜕皮成功。ML1将胆固醇传递给SRB1-12，并为20E的生物合成提供类固醇前体，这反映在全身20E滴度的改变上。ML1和SRB1-12共同调控新发现的基因MLSR的表达，该基因在若虫发育和卵孵化中发挥重要作用。本研究建立了胆固醇在组织内运输的途径，加深了对昆虫体内固醇稳态的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Insect Biochemistry and Molecular Biology 生物-昆虫学

CiteScore

7.40

自引率

5.30%

发文量

105

审稿时长

40 days

期刊介绍： This international journal publishes original contributions and mini-reviews in the fields of insect biochemistry and insect molecular biology. Main areas of interest are neurochemistry, hormone and pheromone biochemistry, enzymes and metabolism, hormone action and gene regulation, gene characterization and structure, pharmacology, immunology and cell and tissue culture. Papers on the biochemistry and molecular biology of other groups of arthropods are published if of general interest to the readership. Technique papers will be considered for publication if they significantly advance the field of insect biochemistry and molecular biology in the opinion of the Editors and Editorial Board.