Comparative analysis of brassica rapa var. cymosa grown in hydroponic condition, lunar maria, and lunar highland regolith simulants: biochemical profiling and effects on drosophila melanogaster as an in vivo model

IF 3.3 3区生物学 Q2 ASTRONOMY & ASTROPHYSICS

Life Sciences in Space Research Pub Date : 2026-01-01 Epub Date: 2025-11-17 DOI:10.1016/j.lssr.2025.11.008

Fatemeh Mansouri , Ana Novo Barros , Laura Bordoni , Donatella Fedeli , Giorgia Pontetti , Eugenia Roncolato , Cinzia Mannozzi , Francesca Pompei , Sauro Vittori , Elena Pettinelli , Natasha Gomes De Miranda , Isabel Gaivão , Rosita Gabbianelli

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引用次数: 0

Abstract

Background

Sustainable crop cultivation in extraterrestrial environments is essential for future space missions. Lunar regolith simulants may influence plant biochemical properties and safety, yet their effects on plant quality and downstream biological responses remain underexplored.

Methods

Brassica rapa var. cymosa was cultivated under hydroponic conditions and in two lunar regolith simulants (lunar maria and lunar highland). Polyphenol profiles, antioxidant capacity, chlorophyll, and carotenoid contents were analyzed. Drosophila melanogaster (Oregon-K) flies were fed media supplemented with five concentrations of each plant sample and a control. DNA damage in larval brain neuroblasts was assessed via comet assay. Adult fly behavior, longevity, and prolificacy were evaluated.

Results

Lunar maria sample showed significantly higher levels of neochlorogenic acid, ferulic acid, and p-coumaric acid, along with increased total phenolic content and chlorophyll a compared to hydroponic and lunar highland samples. Antioxidant capacity, assessed using ferric reducing antioxidant power (FRAP), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays, was higher in lunar-grown plants than in hydroponic ones, whereas oxygen radical absorbance capacity (ORAC) showed no significant difference. DNA damage was increased across all larval groups but was lowest in the lunar maria group at moderate concentrations. Despite early genotoxic stress, adult flies developed normally, with comparable longevity and reproductive output. Notably, flies fed lunar maria samples exhibited enhanced climbing ability.

Conclusion

Cultivation in lunar regolith simulants alters plant biochemistry and induces early DNA damage in drosophila larvae without impairing adult health or behavior. The lunar maria simulant, in particular, promotes beneficial phytochemical traits and organismal resilience. These findings underscore both the challenges and potential of lunar agriculture, emphasizing the critical role of substrate choice in developing sustainable space farming systems.

查看原文本刊更多论文

在水培、月壤和月高地模拟风土条件下生长的芸苔（brassica rapa var. cymosa）的对比分析：生化特征及其对黑腹果蝇（drosophila melanogaster）的影响

地外环境中的可持续作物种植对未来的太空任务至关重要。月球风化模拟物可能影响植物的生化特性和安全性，但其对植物品质和下游生物反应的影响尚不清楚。方法在水培条件下，在两个模拟月球风化层（月壤和月高地）中栽培rapa var. cymosa。分析了多酚谱、抗氧化能力、叶绿素和类胡萝卜素含量。用添加了5种浓度的植物样品和对照的培养基饲喂黑腹果蝇（Oregon-K）。通过彗星试验评估幼脑神经母细胞的DNA损伤。对成虫行为、寿命和繁殖能力进行了评价。结果月壤样品中新绿原酸、阿魏酸和对香豆酸含量显著高于水培样品和月壤样品，总酚和叶绿素a含量显著高于水培样品。通过铁还原抗氧化能力（FRAP）、2,2′-氮基-双（3-乙基苯并噻唑-6-磺酸）（ABTS）和2,2-二苯基-1-吡啶肼（DPPH）测定，月球栽培植物的抗氧化能力高于水培植物，而氧自由基吸收能力（ORAC）无显著差异。在中等浓度下，月牙maria组的DNA损伤程度最低。尽管早期的基因毒性应激，成年果蝇发育正常，具有相当的寿命和生殖产量。值得注意的是，喂食月玛丽亚样本的果蝇表现出增强的攀爬能力。结论月壤模拟物的培养改变了果蝇幼虫的植物生化，诱导了果蝇幼虫早期DNA损伤，但不影响成虫的健康和行为。月球玛丽亚模拟物，特别是，促进有益的植物化学性状和有机体的恢复力。这些发现强调了月球农业的挑战和潜力，强调了基材选择在发展可持续太空农业系统中的关键作用。

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来源期刊

Life Sciences in Space Research Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

5.30

自引率

8.00%

发文量

期刊介绍： Life Sciences in Space Research publishes high quality original research and review articles in areas previously covered by the Life Sciences section of COSPAR''s other society journal Advances in Space Research. Life Sciences in Space Research features an editorial team of top scientists in the space radiation field and guarantees a fast turnaround time from submission to editorial decision.