Highly sensitive chemiluminescence immunoassay utilizing functionalized magnetic metal-organic framework materials for the detection of CEA and CA199

IF 6.1 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2026-03-01 Epub Date: 2026-01-16 DOI:10.1016/j.aca.2026.345114

Xiaomin Zuo , Wenxia Chen , Xianlong Lu , Rui Yuan , Shuangyi Hu , Yangyang Xue , Wei Chen , Rui Yang

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Abstract

Background

In response to the urgent need for highly sensitive and rapid combined detection of multiple tumor markers for the early diagnosis of colorectal cancer, this work innovatively constructs a magnetically-driven chemiluminescence (CL) immunosensing platform (CoFe₂O₄@ZIF-8/luminol-Au NPs).

Results

CoFe₂O₄ NPs were firstly aminated via solvothermal method and lysine modification to provide specific binding sites. ZIF-8 was then grown in situ to form a core-shell structure with high specific surface area for efficient luminol loading. Finally, Au NPs were generated in situ within ZIF-8, simultaneously immobilizing luminol to form composite signal units. This design integrates magnetic separation, confinement effects, and signal amplification, generating an ideal microenvironment for enhanced CL reactions. The label-free CL immunoassay achieves an impressive limit of detection (LOD) of 22 fg/mL for CEA and 5.4 fg/mL for CA199, with broad linear ranges spanning over 5 orders of magnitude. The entire detection process is completed within 30 min, and the analysis of real serum samples demonstrates good recoveries.

Abstract Image

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功能化磁性金属-有机骨架材料的高灵敏度化学发光免疫分析法检测CEA和CA199

为响应结直肠癌早期诊断对多种肿瘤标志物高灵敏度、快速联合检测的迫切需求，本工作创新构建了磁驱动化学发光（CL）免疫传感平台（CoFe2O4@ZIF-8/luminol-Au NPs）。结果首先通过溶剂热法和赖氨酸修饰对scofe2o4 NPs进行胺化，获得了特定的结合位点。然后原位生长ZIF-8，形成具有高比表面积的核壳结构，用于高效负载鲁米诺。最后，在ZIF-8中原位生成Au NPs，同时固定化luminol形成复合信号单元。该设计集成了磁分离、约束效应和信号放大，为增强CL反应提供了理想的微环境。无标记CL免疫分析法的检测限（LOD）令人印象深刻，CEA为22 fg/mL， CA199为5.4 fg/mL，具有超过5个数量级的宽线性范围。整个检测过程在30分钟内完成，对真实血清样品的分析显示出良好的回收率。

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.