Xiaofeng Granules alleviate intrathecal morphine-induced pruritus via inhibiting the NR2B/PKC/CAMK II signaling pathway.

IF 2.8 3区医学 Q2 ANESTHESIOLOGY

Journal of Anesthesia Pub Date : 2026-10-01 Epub Date: 2026-01-17 DOI:10.1007/s00540-026-03663-6

Qiao Wang, Ming Yan, Xu Jing, Jian Sun, Weiqian Tian

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引用次数: 0

Abstract

Purpose: The administration of intrathecal morphine frequently induces pruritus as an adverse reaction. Xiaofeng Granules (XF) is a compound preparation improved from the ancient formula "Xiaofeng Powder". It has been proven to relieve skin itching caused by various reasons in clinical practice. The aim of the study was to evaluate the effect of XF on pruritus caused by intrathecal morphine and possible mechanism.

Methods: Male C57BL/6 mice were intrathecally injected with morphine to induce scratching behavior. The effects of XF on intrathecal morphine-induced pruritus and analgesia were evaluated. The number of scratching response was counted within 30 min after morphine injection. The warm-water tail immersion test was conducted to measure the tail flick latency (TFL) within 120 min after morphine injection. The maximum possible effect percentage (%MPE) and area under the curve (AUC) were calculated based on TFL to evaluate the analgesic effect. Western blot was performed to evaluate the phosphorylation levels of NR2B, PKC and CAMK II in the dorsal horn of the lumbar spinal cord of mice.

Results: Compared with control treatment, intrathecal morphine elicited obvious scratching response when providing analgesic effect in a dose dependent manner. Gavage administration of XF can significantly reduce intrathecal morphine-induced scratching behavior without affecting its analgesic efficiency; besides, XF can inhibit the phosphorylation of NR2B, PKC, and CAMK II induced by intrathecal morphine, which can be reversed by intrathecal injection of NMDA.

Conclusions: XF can relieve intrathecal morphine-induced pruritus and may be related to the inhibition of the NR2B/PKC/CAMK II signaling pathway.

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消风颗粒通过抑制NR2B/PKC/CAMK II信号通路减轻鞘内吗啡引起的瘙痒。

目的：鞘内注射吗啡常引起瘙痒的不良反应。消风颗粒（XF）是由古方“消风散”改良而成的复方制剂。临床实践证明，它能缓解各种原因引起的皮肤瘙痒。本研究的目的是评价XF对鞘内吗啡致瘙痒的作用及其可能的机制。方法：雄性C57BL/6小鼠鞘内注射吗啡诱导抓伤行为。观察XF对鞘内吗啡致瘙痒及镇痛的影响。在吗啡注射后30min内统计抓痒反应次数。采用温水尾浸试验测定吗啡注射后120 min内甩尾潜伏期（TFL）。以TFL为基础计算最大可能效应百分比（%MPE）和曲线下面积（AUC），评价镇痛效果。Western blot检测小鼠腰椎背角NR2B、PKC和CAMK II的磷酸化水平。结果：与对照组相比，鞘内吗啡在提供镇痛作用时可引起明显的抓伤反应，且有剂量依赖性。灌胃XF可显著减少鞘内吗啡引起的抓痕行为，且不影响其镇痛效果；此外，XF可抑制鞘内吗啡诱导的NR2B、PKC和CAMK II的磷酸化，鞘内注射NMDA可逆转这一作用。结论：XF可减轻鞘内吗啡引起的瘙痒，可能与抑制NR2B/PKC/CAMK II信号通路有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Anesthesia 医学-麻醉学

CiteScore

5.30

自引率

7.10%

发文量

112

审稿时长

3-8 weeks

期刊介绍： The Journal of Anesthesia is the official journal of the Japanese Society of Anesthesiologists. This journal publishes original articles, review articles, special articles, clinical reports, short communications, letters to the editor, and book and multimedia reviews. The editors welcome the submission of manuscripts devoted to anesthesia and related topics from any country of the world. Membership in the Society is not a prerequisite. The Journal of Anesthesia (JA) welcomes case reports that show unique cases in perioperative medicine, intensive care, emergency medicine, and pain management.