Advancing pleural effusion diagnosis: Application of Molecular Culture ID in pathogen detection

Abstract

The diagnosis of pleural infection has traditionally relied on microbiological culture, a method often limited by slow bacterial growth and difficulties in culturing fastidious organisms. This study introduces Molecular Culture ID, a rapid and comprehensive molecular technique capable of detecting and identifying a wide range of bacteria down to the species level. By analyzing 440 pleural effusion samples derived from patients suspected of pleural infection and comparing the outcome to routine diagnostics, we demonstrate that Molecular Culture ID significantly outperforms traditional culture. Molecular Culture ID identified 133 positive samples, compared to 55 positive samples identified by traditional culture. The 78 additional positive samples detected by Molecular Culture contained important pathogens including species such as Streptococcus pneumoniae, Escherichia coli, and Staphylococcus aureus. These findings highlight Molecular Culture ID as a promising alternative for the rapid and accurate diagnosis of pleural infections, offering a substantial improvement over conventional methods.