Kenan Ç Tümer, Tarık Şafak, İsa Şekercioğlu, Alper O Akbaş
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引用次数: 0
Abstract
Background: The periparturient period in dairy cows is marked by negative energy balance (NEB), resulting in metabolic stress and an increased risk of postpartum diseases. Accurate assessment of NEB through blood β-hydroxybutyrate (β-OHB) levels is essential for effective herd management. Point-of-care (POC) devices, such as the CentriVet blood ketone monitoring system, offer a practical alternative to laboratory methods for measuring β-OHB.
Objective: This study aimed to evaluate the diagnostic and analytical performance of the CentriVet blood ketone monitoring system compared to a laboratory reference method for measuring blood β-OHB concentration in postpartum dairy cattle.
Methods: A total of 105 postpartum dairy cows from two farms were included. Blood samples were analyzed for β-OHB using both CentriVet and a reference enzymatic method. Passing-Bablok regression, Bland-Altman plots, and kappa statistics were used to assess agreement. Diagnostic accuracy metrics (sensitivity, specificity, PPV, NPV, and accuracy) were calculated at a cut-off value of ≥ 1.2 mmol/L. An optimized cut-off value was determined via ROC curve analysis.
Results: CentriVet demonstrated a moderate correlation with the reference method (Spearman's r = 0.701, p < 0.001). Passing-Bablok regression revealed proportional bias, and Bland-Altman analysis indicated a mean bias of -0.4 mmol/L. Diagnostic metrics at ≥ 1.2 mmol/L showed 100% sensitivity, 68.09% specificity, and 71.4% accuracy. ROC analysis yielded an optimized cut-off of > 1.5 mmol/L, improving specificity (95.7%) but reducing sensitivity (81.8%).
Conclusions: While CentriVet offers practical benefits, its diagnostic performance is limited for accurately identifying hyperketonemia in postpartum dairy cattle.
背景：奶牛围产期存在负能量平衡（NEB），导致代谢应激，产后疾病风险增加。通过血液β-羟基丁酸（β-OHB）水平准确评估NEB对于有效的牛群管理至关重要。护理点（POC）设备，如centrvet血酮监测系统，为测量β-OHB提供了一种实用的替代实验室方法。目的：本研究旨在评价centrvet血酮监测系统与实验室参考法测定产后奶牛血液中β-OHB浓度的诊断和分析性能。方法：选取两个农场的105头产后奶牛。采用centrvet和参考酶法分析血样中β-OHB的含量。使用passingbablok回归、Bland-Altman图和kappa统计来评估一致性。诊断准确性指标（敏感性、特异性、PPV、NPV和准确性）以≥1.2 mmol/L的临界值计算。通过ROC曲线分析确定最佳临界值。结果：centrvet与参比方法相关性中等（Spearman’s r = 0.701, p 1.5 mmol/L），特异性提高（95.7%），敏感性降低（81.8%）。结论：虽然centrvet提供了实际的好处，但其诊断性能在准确识别产后奶牛高酮血症方面是有限的。
期刊介绍：
Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.
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