Fine mapping and cloning of an intersubspecific reproductive isolation locus MSHB1 in rice.

IF 3.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2026-01-13 eCollection Date: 2026-01-01 DOI:10.1007/s11032-025-01630-9

Xuan Wang, Zhengjiu Zhang, Jinfeng Zhao, Mengjiao Chen, Junyi Gong, Yu Han, Xin Wei, Xuehui Huang, Jiongjiong Fan

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Abstract

Reproductive isolation between rice subspecies poses a significant barrier to harnessing heterosis in hybrid breeding. Here, we identified and characterized a novel locus, designated Male Sterility-Related Hybrid Breakdown Locus on Chromosome 1 (MSHB1), through the utilization of chromosome segment substitution lines (CSSLs) derived from crosses between an indica cultivar Huanghuazhan (HHZ) and an aromatic cultivar Basmati Surkh 89 - 15 (BAS). A near-isogenic line, CS004, carrying the BAS-derived MSHB1 ^BAS allele, exhibits complete male sterility, reduced plant height, fewer panicles, and shorter panicle length. Fine mapping localized MSHB1 to a 28.583-kb interval on the short arm of chromosome 1. Genetic complementation confirmed that the MSHB1 allele from HHZ is sufficient to restore fertility. MSHB1 encodes a homolog of Esa1-associated factor 6 (EAF6), which regulates histone acetylation and transcriptional activity. Comparative genomic analyses revealed that functional divergence in promoter activity between duplicated OsEAF6 paralogs (MSHB1 and MSHB12) drives hybrid breakdown. These findings elucidate a molecular mechanism underlying intersubspecific reproductive isolation and highlight MSHB1 as a strategic target for overcoming hybrid sterility to enhance heterosis in rice breeding.

Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01630-9.

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水稻亚种间生殖隔离位点MSHB1的精细定位与克隆。

水稻亚种间的生殖隔离是杂种优势利用的重要障碍。本研究利用籼稻品种黄花藏（HHZ）与芳香品种巴斯马蒂89 - 15 （BAS）杂交的染色体片段代换系（CSSLs），鉴定并鉴定了1号染色体上一个新的雄性不育相关杂交破坏位点（MSHB1）。近等基因系CS004携带BAS衍生的MSHB1 BAS等位基因，表现出完全雄性不育，株高降低，穗数减少，穗长缩短。在1号染色体短臂上精细定位MSHB1至28.583 kb区间。遗传互补证实来自HHZ的MSHB1等位基因足以恢复生育能力。MSHB1编码esa1相关因子6 （EAF6）的同源物，EAF6调节组蛋白乙酰化和转录活性。比较基因组分析显示，重复的OsEAF6相似物（MSHB1和MSHB12）之间启动子活性的功能差异驱动杂交破坏。这些发现阐明了亚种间生殖隔离的分子机制，并强调MSHB1是水稻育种中克服杂种不育、增强杂种优势的战略靶点。补充资料：在线版本提供补充资料，网址为10.1007/s11032-025-01630-9。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.