Optimization of a Method for Measuring 2,3-Diphosphoglycerate in Erythrocytes Using 31P NMR Analysis

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2026-01-14 DOI:10.1134/S0006350925700630

M. V. Molchanov, A. Ye. Yegorov, A. V. Kholina, E. A. Kalabina, N. A. Borozdina, S. I. Baydarova, M. S. Kazakova, E. V. Arshintseva, S. Yu. Pushkin, M. A. Timchenko

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Abstract

2,3-Diphosphoglycerate is present in large quantities in mammalian red blood cells and controls the affinity of hemoglobin to oxygen. The level of 2,3-diphosphoglycerate changes with the development of certain pathologies, such as chronic lung diseases, anemia, and hyperthyroidism. 31P NMR spectroscopy has shown that reducing the number of washings of rat erythrocytes significantly increases the stability of 2,3-diphosphoglycerate in these samples. The level of 2,3-diphosphoglycerate does not change for 3.5 h with a single wash and for 4 h without washing. At the same time, with standard double washing, the content of 2,3-diphosphoglycerate decreases after 2.5 h. Analysis of its distribution between red blood cells and removed plasma, as well as analysis of the serum of one animal, showed that all the 2,3-diphosphoglycerate remains in the suspension of red blood cells and is absent in the plasma and serum. The results we obtained should be taken into account when working with intact erythrocytes.

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用31P核磁共振分析测定红细胞中2,3-二磷酸甘油酸的方法优化

2,3-二磷酸甘油酸在哺乳动物红细胞中大量存在，并控制血红蛋白对氧的亲和力。2,3-二磷酸甘油酸水平随着某些病理的发展而变化，如慢性肺病、贫血和甲状腺功能亢进。31P核磁共振波谱显示，减少大鼠红细胞的洗涤次数显著增加了2,3-二磷酸甘油酸在这些样品中的稳定性。2,3-二磷酸甘油酸的水平在单次洗涤3.5小时和不洗涤4小时内没有变化。同时，采用标准的二次洗涤，2.5 h后2,3-二磷酸甘油酸的含量降低。分析其在红细胞和去除的血浆之间的分布，以及对一只动物的血清的分析表明，2,3-二磷酸甘油酸全部保留在红细胞悬浮液中，而在血浆和血清中不存在。在处理完整红细胞时，我们得到的结果应予以考虑。

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.