A. G. Mironova, B. V. Trubitsin, E. Yu. Simonenko, A. V. Sybachin, S. A. Yakovenko, A. N. Tikhonov
{"title":"The Effect of Cholesterol on Spermatozoa Membranes: Investigation Using Spin Probes","authors":"A. G. Mironova, B. V. Trubitsin, E. Yu. Simonenko, A. V. Sybachin, S. A. Yakovenko, A. N. Tikhonov","doi":"10.1134/S0006350925700678","DOIUrl":null,"url":null,"abstract":"<p>Cholesterol plays an important role in the structural organization and functioning of spermatozoa membranes. The purpose of this work is to study the structural changes in human spermatozoa membranes using spin probes after removing cholesterol from them using beta-methylcyclodextrin, which binds cholesterol. Using a model system (liposomes with different cholesterol content), it was shown that the presence of cholesterol in liposomes affected the EPR spectra of the spin probe 5-doxyl stearate dissolved in the lipid bilayer, causing an increase in the microviscosity of the membrane. A study performed on human spermatozoa showed that the extraction of cholesterol from them using beta-methylcyclodextrin was accompanied by an increase in the mobility of the spin probe 5-doxyl stearate, indicating that the removal of cholesterol helps to reduce the microviscosity of the lipid bilayer of spermatozoa membranes.</p>","PeriodicalId":493,"journal":{"name":"Biophysics","volume":"70 4","pages":"632 - 642"},"PeriodicalIF":4.0330,"publicationDate":"2026-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysics","FirstCategoryId":"4","ListUrlMain":"https://link.springer.com/article/10.1134/S0006350925700678","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Cholesterol plays an important role in the structural organization and functioning of spermatozoa membranes. The purpose of this work is to study the structural changes in human spermatozoa membranes using spin probes after removing cholesterol from them using beta-methylcyclodextrin, which binds cholesterol. Using a model system (liposomes with different cholesterol content), it was shown that the presence of cholesterol in liposomes affected the EPR spectra of the spin probe 5-doxyl stearate dissolved in the lipid bilayer, causing an increase in the microviscosity of the membrane. A study performed on human spermatozoa showed that the extraction of cholesterol from them using beta-methylcyclodextrin was accompanied by an increase in the mobility of the spin probe 5-doxyl stearate, indicating that the removal of cholesterol helps to reduce the microviscosity of the lipid bilayer of spermatozoa membranes.
BiophysicsBiochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍：
Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
