Histological Comparison of Bone Regeneration Using Two Putty Bone Grafts in Surgically Created Intrabony Defect in Rats with Intramarrow Penetration.

Q3 Dentistry

Journal of Contemporary Dental Practice Pub Date : 2025-10-01 DOI:10.5005/jp-journals-10024-3953

Afaf M Dao, Mohamed Abdulrahman, Eman S Mira, Islam Kandil, Mohamed A Al-Shahat

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引用次数: 0

Abstract

Aim of study: This study aimed to histologically evaluate and compare the bone regenerative potential of two grafting materials, demineralized bone matrix (DBM) putty and nanocrystalline hydroxyapatite (ncHA) putty, applied to surgically created intrabony defects in a rat's model, in conjunction with the decortication technique.

Materials and methods: Thirty rats were included. A surgical bone defect measuring W × L × D; 2 × 2 × 1.5 mm with decortication was prepared by small round bur, and the rats were randomly divided into three groups (10 rats each), group I (control): Defect induction and decortication, group II: Defect induction and decortication then, ncHA putty insertion, group III: Defect induction and decortication then DBM allograft putty insertion. Five rats from each group were euthanized at 2 and 4 weeks, respectively. Maxilla excised for histological processing and evaluation using H&E stains to assess the percentage area of newly formed bone. Data were statistically analyzed by one-way ANOVA and post hoc test.

Results: The mean ± SD values at 2 and at 4 weeks were (1.05 ± 0.07, 1.25 ± 0.04) for groups I, group II (8.14 ± 0.16, 15.94 ± 0.21), and group III (8.20 ± 0.25, 13.40 ± 0.55), respectively. Both experimental groups showed significantly greater bone formation than the control at both intervals (p < 0.001). At 4 weeks, group II (ncHA) recorded higher bone regeneration compared to group III DBM (p < 0.001), while the control group showed only a slight increase.

Conclusion: Both DBM and ncHA putties enhanced bone regeneration, with ncHA showing superior performance at 4 weeks.

Clinical significance: Intrabony defects are a clinical challenge, and selecting an effective graft material is crucial for achieving predictable periodontal regeneration. How to cite this article: Dao AM, Abdulrahman M, Mira ES, et al. Histological Comparison of Bone Regeneration Using Two Putty Bone Grafts in Surgically Created Intrabony Defect in Rats with Intramarrow Penetration. J Contemp Dent Pract 2025;26(10):938-944.

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两种骨灰移植修复大鼠动脉内穿透骨缺损的组织学比较。

研究目的：本研究旨在组织学上评估和比较两种移植材料，脱矿骨基质（DBM）腻子和纳米晶羟基磷灰石（ncHA）腻子的骨再生潜力，并结合去皮技术应用于大鼠骨内手术缺损模型。材料与方法：大鼠30只。外科骨缺损测量W × L × D；采用小圆榫制备2 × 2 × 1.5 mm去皮大鼠，随机分为3组（每组10只），ⅰ组（对照组）：缺陷诱导后去皮，ⅱ组：缺陷诱导后去皮，再插入ncHA灰，ⅲ组：缺陷诱导后去皮，再插入DBM同种异体骨灰。每组5只大鼠分别于第2周和第4周实施安乐死。切除上颌骨进行组织学处理，并用H&E染色法评估新生骨面积百分比。数据采用单因素方差分析和事后检验进行统计学分析。结果：ⅰ组（8.14±0.16），ⅱ组（15.94±0.21），ⅲ组（8.20±0.25,13.40±0.55）的平均±SD值分别为（1.05±0.07,1.25±0.04）。两个实验组的骨形成均显著高于对照组（p < 0.001）。在第4周，与第III组相比，第II组（ncHA）记录了更高的骨再生（p < 0.001），而对照组仅略有增加。结论：DBM和ncHA均能促进骨再生，其中ncHA在第4周表现更佳。临床意义：骨内缺损是一个临床挑战，选择有效的移植材料对于实现可预测的牙周再生至关重要。本文引用方式：Dao AM， Abdulrahman M, Mira ES，等。两种骨灰移植修复大鼠动脉内穿透骨缺损的组织学比较。[J]现代医学学报；2009;26(10):938-944。

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来源期刊

Journal of Contemporary Dental Practice Dentistry-Dentistry (all)

CiteScore

1.80

自引率

0.00%

发文量

174

期刊介绍： The Journal of Contemporary Dental Practice (JCDP), is a peer-reviewed, open access MEDLINE indexed journal. The journal’s full text is available online at http://www.thejcdp.com. The journal allows free access (open access) to its contents. Articles with clinical relevance will be given preference for publication. The Journal publishes original research papers, review articles, rare and novel case reports, and clinical techniques. Manuscripts are invited from all specialties of dentistry i.e., conservative dentistry and endodontics, dentofacial orthopedics and orthodontics, oral medicine and radiology, oral pathology, oral surgery, orodental diseases, pediatric dentistry, implantology, periodontics, clinical aspects of public health dentistry, and prosthodontics.