Synergistic effects of zinc oxide and ellagic acid on macrophage infiltration, angiogenesis, and collagen deposition in excisional wounds healing

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2026-03-01 Epub Date: 2026-01-13 DOI:10.1016/j.jobcr.2026.01.002

Intan Nirwana , Michelle Fidelia Alexandra , Tarissa Balqis Nuraida , Ahmad Thariq Bermani , Yasmin Shahaniz , Devi Rianti , Titien Hary Agustantina , Anita Yuliati , Khairul Annuar Shariff , Meircurius Dwi Condro Surboyo

{"title":"Synergistic effects of zinc oxide and ellagic acid on macrophage infiltration, angiogenesis, and collagen deposition in excisional wounds healing","authors":"Intan Nirwana , Michelle Fidelia Alexandra , Tarissa Balqis Nuraida , Ahmad Thariq Bermani , Yasmin Shahaniz , Devi Rianti , Titien Hary Agustantina , Anita Yuliati , Khairul Annuar Shariff , Meircurius Dwi Condro Surboyo","doi":"10.1016/j.jobcr.2026.01.002","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>The combination of zinc oxide (ZnO) and ellagic acid (EA) in wound dressings offers synergistic benefits for wound healing due to their complementary antimicrobial, antioxidant, anti-inflammatory, and tissue regenerative properties. This study aimed to evaluate the effects of ZnO-EA dressing on macrophage infiltration, angiogenesis, and collagen density in excisional wounds on days 3 and 7 post-treatment.</div></div><div><h3>Materials and methods</h3><div>This in vivo posttest-only experimental study involved twenty <em>Rattus norvegicus</em> with excisional dorsal wounds, randomly assigned to two groups: a control group receiving ZnO dressing and a treatment group receiving ZnO-EA dressing. Each group was evaluated at day 3 and day 7, resulting in four observation subgroups. Tissue samples were collected to assess macrophage infiltration and angiogenesis using Hematoxylin and Eosin staining, and collagen density using Masson's Trichrome staining. Histological evaluation was performed under a light microscope at 400× magnification.</div></div><div><h3>Results</h3><div>Treatment with ZnO-EA resulted in significant increase in macrophages infiltration, the number of newly formed blood vessels, and collagen density in the treatment group compared to the ZnO group on both days 3 and 7 (p < 0.05).</div></div><div><h3>Conclusion</h3><div>The application of a ZnO wound dressing enriched with EA significantly enhances macrophage infiltration, angiogenesis, and collagen deposition during the wound healing process.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"16 2","pages":"Article 101398"},"PeriodicalIF":0.0000,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral biology and craniofacial research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212426826000023","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/1/13 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives

The combination of zinc oxide (ZnO) and ellagic acid (EA) in wound dressings offers synergistic benefits for wound healing due to their complementary antimicrobial, antioxidant, anti-inflammatory, and tissue regenerative properties. This study aimed to evaluate the effects of ZnO-EA dressing on macrophage infiltration, angiogenesis, and collagen density in excisional wounds on days 3 and 7 post-treatment.

Materials and methods

This in vivo posttest-only experimental study involved twenty Rattus norvegicus with excisional dorsal wounds, randomly assigned to two groups: a control group receiving ZnO dressing and a treatment group receiving ZnO-EA dressing. Each group was evaluated at day 3 and day 7, resulting in four observation subgroups. Tissue samples were collected to assess macrophage infiltration and angiogenesis using Hematoxylin and Eosin staining, and collagen density using Masson's Trichrome staining. Histological evaluation was performed under a light microscope at 400× magnification.

Results

Treatment with ZnO-EA resulted in significant increase in macrophages infiltration, the number of newly formed blood vessels, and collagen density in the treatment group compared to the ZnO group on both days 3 and 7 (p < 0.05).

Conclusion

The application of a ZnO wound dressing enriched with EA significantly enhances macrophage infiltration, angiogenesis, and collagen deposition during the wound healing process.

查看原文本刊更多论文

氧化锌和鞣花酸对切除伤口愈合中巨噬细胞浸润、血管生成和胶原沉积的协同作用

目的将氧化锌（ZnO）和鞣花酸（EA）结合在伤口敷料中，具有抗菌、抗氧化、抗炎和组织再生的协同作用。本研究旨在评价ZnO-EA敷料在治疗后第3天和第7天对切除创面巨噬细胞浸润、血管生成和胶原蛋白密度的影响。材料与方法本实验选取20只背部切除创面的褐家鼠，随机分为两组：对照组和治疗组分别采用ZnO- ea敷料。每组在第3天和第7天进行评估，形成4个观察亚组。收集组织样本，用苏木精和伊红染色评估巨噬细胞浸润和血管生成，用马松三色染色评估胶原蛋白密度。光镜下400倍放大进行组织学观察。结果与ZnO组相比，ZnO- ea治疗组在第3天和第7天巨噬细胞浸润、新生血管数量和胶原蛋白密度均显著增加（p < 0.05）。结论添加EA的氧化锌创面敷料可显著增强创面愈合过程中巨噬细胞的浸润、血管生成和胶原的沉积。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.