Promising diagnostic performance of a commercial qPCR assay for Plasmodium vivax detection: Contributing to malaria control and elimination

IF 2.5 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of microbiological methods Pub Date : 2026-02-01 Epub Date: 2026-01-07 DOI:10.1016/j.mimet.2026.107391

Marcelo Cerilo-Filho , Marrara P. Sampaio , Maria Naely G. Almeida , Amanda A. Silva , Gabriel Montoia , Luciane M. Storti-Melo , Andréa Regina S. Baptista , Silvia Maria Di Santi , Ricardo Luiz D. Machado

引用次数: 0

Abstract

This study evaluated a commercial qPCR assay for P. vivax detection. Among 167 samples, the assay showed greater sensitivity than microscopy, detecting three additional positives. It also demonstrated high specificity and no cross-reactivity. Our results support its value as a reliable tool for malaria diagnosis and surveillance.

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间日疟原虫检测的商业qPCR检测有前景的诊断性能：有助于疟疾的控制和消除

本研究评估了一种用于间日疟原虫检测的商用qPCR检测方法。在167个样本中，该分析显示出比显微镜更高的灵敏度，检测到三个额外的阳性。它还显示出高特异性和无交叉反应性。我们的结果支持它作为疟疾诊断和监测的可靠工具的价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.