Amelioration of mining-area soils with microbial induction of magnesium ammonium phosphate

IF 3.6 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biochemical Engineering Journal Pub Date : 2026-04-01 Epub Date: 2026-01-07 DOI:10.1016/j.bej.2026.110075

Zhe Wang , Wen-yan Zhang , Jun-li Cheng , Shu-hui Wei , Hong-yuan Cui , Wei-da Wang

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引用次数: 0

Abstract

Large-scale mineral exploitation causes heavy metal pollution and nutrient depletion in mining-area soils, making heavy metal control and soil fertility improvement urgent. This study synthesized biological magnesium ammonium phosphate (bio-MAP) via microbially induced mineralization, characterized its microstructure, and conducted pot experiments with ryegrass and alfalfa under different bio-MAP additions to analyze effects on soil physicochemical properties, Pb²⁺/Cd²⁺ contents, and plant growth. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) confirmed the precipitate was mainly struvite (MgNH₄PO₄·6H₂O) with a regular orthorhombic structure. Appropriate bio-MAP addition not only facilitated the formation of large soil aggregates and enhanced soil structural stability but also significantly promoted the growth of ryegrass and alfalfa. At the 30-day harvest, the total fresh weight of ryegrass reached 1.33 g in the P5 treatment (bio-MAP addition: 1000 mg/kg), while that of alfalfa reached 2.36 g in the P3 treatment (bio-MAP addition: 500 mg/kg). Compared with the control, the P1, P3, and P5 treatments reduced soil available Pb content by 7.16 %, 15.1 %, and 19.28 %, respectively, and soil available Cd content by 58.57 %, 62.31 %, and 65.1 %, respectively. Bio-MAP also increased soil sucrase activity and abundances of Proteobacteria, Actinobacteria, and Bacillus. This study provides a new method for mine soil amendment and heavy metal control, plus references for ecological restoration.

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微生物诱导磷酸铵镁改良矿区土壤

大规模矿产开采造成矿区土壤重金属污染和养分耗竭，治理重金属和提高土壤肥力刻不容缓。本研究通过微生物诱导矿化法合成了生物磷酸镁铵（bio-MAP），对其微观结构进行了表征，并在不同生物map添加量的黑麦草和苜蓿上进行了盆栽实验，分析了生物map添加量对土壤理化性质、Pb 2 + /Cd 2 +含量以及植物生长的影响。x射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和扫描电镜（SEM）证实沉淀主要为鸟粪石（MgNH₄PO₄·6H₂O），具有规则的正交结构。适当添加生物map不仅有利于土壤团聚体的形成，增强土壤结构稳定性，而且显著促进黑麦草和苜蓿的生长。收获30 d时，P5处理（bio-MAP添加量为1000 mg/kg）黑麦草总鲜重达到1.33 g， P3处理（bio-MAP添加量为500 mg/kg）紫花苜蓿总鲜重达到2.36 g。与对照相比，P1、P3和P5处理土壤有效Pb含量分别降低了7.16 %、15.1 %和19.28 %，土壤有效Cd含量分别降低了58.57 %、62.31 %和65.1% %。Bio-MAP还增加了土壤蔗糖酶活性和变形菌、放线菌和芽孢杆菌的丰度。该研究为矿山土壤修复和重金属控制提供了新的方法，并为生态修复提供了参考。

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来源期刊

Biochemical Engineering Journal 工程技术-工程：化工

CiteScore

7.10

自引率

5.10%

发文量

380

审稿时长

34 days

期刊介绍： The Biochemical Engineering Journal aims to promote progress in the crucial chemical engineering aspects of the development of biological processes associated with everything from raw materials preparation to product recovery relevant to industries as diverse as medical/healthcare, industrial biotechnology, and environmental biotechnology. The Journal welcomes full length original research papers, short communications, and review papers* in the following research fields: Biocatalysis (enzyme or microbial) and biotransformations, including immobilized biocatalyst preparation and kinetics Biosensors and Biodevices including biofabrication and novel fuel cell development Bioseparations including scale-up and protein refolding/renaturation Environmental Bioengineering including bioconversion, bioremediation, and microbial fuel cells Bioreactor Systems including characterization, optimization and scale-up Bioresources and Biorefinery Engineering including biomass conversion, biofuels, bioenergy, and optimization Industrial Biotechnology including specialty chemicals, platform chemicals and neutraceuticals Biomaterials and Tissue Engineering including bioartificial organs, cell encapsulation, and controlled release Cell Culture Engineering (plant, animal or insect cells) including viral vectors, monoclonal antibodies, recombinant proteins, vaccines, and secondary metabolites Cell Therapies and Stem Cells including pluripotent, mesenchymal and hematopoietic stem cells; immunotherapies; tissue-specific differentiation; and cryopreservation Metabolic Engineering, Systems and Synthetic Biology including OMICS, bioinformatics, in silico biology, and metabolic flux analysis Protein Engineering including enzyme engineering and directed evolution.