Stability and Perpetuation of Human T-Lymphotropic Virus 1 (HTLV-1) in Intrafamilial Transmissions of Infection in an Endemic Region of the Brazilian Amazon: A Pilot Study.

IF 2.6 4区医学 Q3 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH

Journal of Tropical Medicine Pub Date : 2025-12-29 eCollection Date: 2025-01-01 DOI:10.1155/jotm/2728551

Cássia Cristine Costa Pereira, Milena Cristina Martins da Silva, Samir Mansour Moraes Casseb, Maria de Nazaré Lima Dos Reis, Louise de Souza Canto Covre, Edna Aoba Yassui Ishikawa, Leonardo Miranda Dos Santos, Carlos Araújo da Costa, Maisa Silva de Sousa

{"title":"Stability and Perpetuation of Human T-Lymphotropic Virus 1 (HTLV-1) in Intrafamilial Transmissions of Infection in an Endemic Region of the Brazilian Amazon: A Pilot Study.","authors":"Cássia Cristine Costa Pereira, Milena Cristina Martins da Silva, Samir Mansour Moraes Casseb, Maria de Nazaré Lima Dos Reis, Louise de Souza Canto Covre, Edna Aoba Yassui Ishikawa, Leonardo Miranda Dos Santos, Carlos Araújo da Costa, Maisa Silva de Sousa","doi":"10.1155/jotm/2728551","DOIUrl":null,"url":null,"abstract":"Introduction: Human T-lymphotropic virus 1 (HTLV-1) is related to several clinical diseases, including adult T-cell leukemia/lymphoma and HTLV-1-associated myelopathy. Intrafamilial transmission of HTLV-1 is rarely reported in Brazil and worldwide.Objective: To identify HTLV-1 family transmissions in Belem, Para, the Amazon region of Brazil.Methods: Nested PCR of the pX gene, followed by restrict fragment length polymorphism (RFLP) to identify HTLV-1. Nested PCR of the 5'LTR region was used for nucleotide sequencing. Nucleotide sequences were analyzed using GENEIOUS 4.8.5 and MAFFT V.7.Results: The study investigated 72 individuals (14 index cases and 58 relatives) from 14 families, identifying HTLV-1 infection in 47% (27/58) of the relatives. Vertical transmission occurred in 14 (39%) of the 36 mother/child relationships investigated, and sexual transmission was observed in 14 (74%) of the 19 marital relationships (p = 0.0299). Both transmission routes were identified only in families with the highest average number of relatives investigated. Nucleotide analysis demonstrated HTLV-1 stability in intrafamilial transmission.Conclusion: High rate of HTLV-1 intrafamilial transmission was identified in this study. Sexual and vertical transmission are important to familiar dissemination and perpetuation of the virus in this endemic area of the Brazilian Amazon.","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"2025 ","pages":"2728551"},"PeriodicalIF":2.6000,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12747787/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Tropical Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/jotm/2728551","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction: Human T-lymphotropic virus 1 (HTLV-1) is related to several clinical diseases, including adult T-cell leukemia/lymphoma and HTLV-1-associated myelopathy. Intrafamilial transmission of HTLV-1 is rarely reported in Brazil and worldwide.

Objective: To identify HTLV-1 family transmissions in Belem, Para, the Amazon region of Brazil.

Methods: Nested PCR of the pX gene, followed by restrict fragment length polymorphism (RFLP) to identify HTLV-1. Nested PCR of the 5'LTR region was used for nucleotide sequencing. Nucleotide sequences were analyzed using GENEIOUS 4.8.5 and MAFFT V.7.

Results: The study investigated 72 individuals (14 index cases and 58 relatives) from 14 families, identifying HTLV-1 infection in 47% (27/58) of the relatives. Vertical transmission occurred in 14 (39%) of the 36 mother/child relationships investigated, and sexual transmission was observed in 14 (74%) of the 19 marital relationships (p = 0.0299). Both transmission routes were identified only in families with the highest average number of relatives investigated. Nucleotide analysis demonstrated HTLV-1 stability in intrafamilial transmission.

Conclusion: High rate of HTLV-1 intrafamilial transmission was identified in this study. Sexual and vertical transmission are important to familiar dissemination and perpetuation of the virus in this endemic area of the Brazilian Amazon.

Abstract Image

查看原文本刊更多论文

人类嗜t淋巴病毒1 （HTLV-1）在巴西亚马逊地区家族内传播感染中的稳定性和持久性：一项试点研究。

人类嗜t淋巴病毒1 （HTLV-1）与多种临床疾病有关，包括成人t细胞白血病/淋巴瘤和HTLV-1相关的脊髓病。HTLV-1家族内传播在巴西和世界范围内很少报道。目的：确定HTLV-1家族在巴西贝伦、帕拉和亚马逊地区的传播。方法：对pX基因进行巢式PCR，然后采用限制性片段长度多态性（RFLP）对HTLV-1进行鉴定。采用5'LTR区巢式PCR进行核苷酸测序。核苷酸序列分析采用genus4.8.5和matfft V.7。结果：本研究共调查了14个家庭的72人（14例指示病例和58例亲属），其中47%（27/58）的亲属感染了HTLV-1。在调查的36个母子关系中，有14个（39%）发生垂直传播，19个婚姻关系中有14个（74%）发生性传播（p = 0.0299）。仅在调查亲属平均人数最多的家庭中发现了这两种传播途径。核苷酸分析显示HTLV-1在家族内传播中的稳定性。结论：本研究发现HTLV-1家族内传播率高。性传播和垂直传播对该病毒在巴西亚马逊地区的常见传播和延续很重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Tropical Medicine Immunology and Microbiology-Parasitology

CiteScore

3.90

自引率

4.50%

发文量

审稿时长

14 weeks

期刊介绍： Journal of Tropical Medicine is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on all aspects of tropical diseases. Articles on the pathology, diagnosis, and treatment of tropical diseases, parasites and their hosts, epidemiology, and public health issues will be considered. Journal of Tropical Medicine aims to facilitate the communication of advances addressing global health and mortality relating to tropical diseases.