Similar global transcription patterns in mouse lung tissue following pulmonary exposure to renewable and conventional diesel engine exhaust particles

IF 4.2 3区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Environmental toxicology and pharmacology Pub Date : 2026-03-01 Epub Date: 2025-12-26 DOI:10.1016/j.etap.2025.104918

Anda R. Gliga , Sarah McCarrick , Vilhelm Malmborg , Pekka Kohonen , Anastasiia Snigireva , Brandon Mills , Pernille Høgh Danielsen , Lena Palmberg , Karin Broberg , Joakim Pagels , Ulla Vogel

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Abstract

Renewable diesel fuels may reduce greenhouse gas emissions and particulate matter compared to fossil diesel, but the toxicity of their combustion products remains unclear. This study assessed pulmonary effects of renewable diesel exhaust particles in female C57BL/6NTac mice after single intratracheal instillation (6, 18, or 54 µg/mouse). Particles were generated from renewable fuels (rapeseed methyl ester, RME; hydrogen-treated vegetable oil, HVO) and petroleum diesel (DEP) using a modern heavy-duty diesel engine. Lung tissue was analysed via RNA sequencing one day post-exposure to identify differentially expressed genes, followed by pathway analysis and benchmark dose (BMD) modelling. Enriched pathways revealed similar toxicological profiles across fuels, involving immune response, extracellular matrix, and cardiovascular signalling. Pathway activation scores and BMDs indicated that HVO and DEP have similar pro-inflammatory potencies whereas RME was less potent. In conclusion, the similarity of the toxicological responses for renewable and traditional diesel exhaust particles raises health concerns for renewable diesels.

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小鼠肺组织暴露于可再生和传统柴油发动机排气颗粒后相似的全球转录模式

与化石柴油相比，可再生柴油可能会减少温室气体排放和颗粒物，但其燃烧产物的毒性尚不清楚。本研究评估了可再生柴油废气颗粒在雌性C57BL/6NTac小鼠单次气管内灌注（6、18或54 µg/小鼠）后对肺部的影响。颗粒由可再生燃料（油菜籽甲酯，RME；氢处理植物油，HVO）和使用现代重型柴油发动机的石油柴油（DEP）产生。暴露后1天通过RNA测序分析肺组织以鉴定差异表达基因，随后进行途径分析和基准剂量（BMD）建模。丰富的通路在不同燃料中显示出相似的毒理学特征，包括免疫反应、细胞外基质和心血管信号。通路激活评分和bmd表明HVO和DEP具有相似的促炎效力，而RME的效力较弱。综上所述，可再生能源和传统柴油废气颗粒的毒理学反应的相似性引起了对可再生柴油的健康关注。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Environmental toxicology and pharmacology 医学-毒理学

CiteScore

7.00

自引率

4.70%

发文量

185

审稿时长

34 days

期刊介绍： Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man. Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals. In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.