A dual-signal electrochemical aptasensor based on rGO@MoS2-Fc nanozyme for sensitive detection of low-density lipoprotein

IF 4.9 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2026-06-01 Epub Date: 2025-12-24 DOI:10.1016/j.bioelechem.2025.109206

Zhide Zhou , Haimei Li , Xiaohua Deng , Yuxuan Shi , Jiahang Huang , Yu Zhou , Xiaohong Tan , Jintao Liang , Guiyin Li

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引用次数: 0

Abstract

Cardiovascular disease (CVD) is the leading cause of death worldwide, particularly in the developing countries. Low-density lipoprotein (LDL), a critical biomarker for the early detection and intervention of CVD, provides valuable diagnostic and prognostic information for CVD. Herein, we developed a dual-signal electrochemical aptasensor for LDL detection based on a peroxidase-like nanozyme composed of reduced graphene oxide@molybdenum disulfide-ferrocenecarboxylic acid (rGO@MoS₂-Fc). In the process of electrochemical testing, rGO@MoS₂-Fc achieved the synergistic amplification of electrochemical signals both an enhanced Fc oxidation current detected by differential pulse voltammetry (DPV), and an increased hydrogen peroxide (H₂O₂) decomposition current via amperometric current-time (i-t) analysis. The DPV signal, corresponding to Fc oxidation, exhibited a logarithmic linear response over a broad LDL concentration ranged from 0.001 to 100.0 μg/mL, with a limit of detection (LOD) as low as 0.91 ng/mL. Meanwhile, the i-t signal, arising from H₂O₂ decomposition, displayed a linear range of 1.0 to 80.0 μg/mL and an LOD of 0.80 μg/mL. This dual-signal strategy exhibited good selectivity, reproducibility, stability and self-calibration capability. Analysis of human serum samples showed that the proposed strategy is expected to be a powerful method for early-stage CVD diagnosis and clinical management.

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基于rGO@MoS2-Fc纳米酶的双信号电化学感应传感器用于低密度脂蛋白的灵敏检测

心血管疾病（CVD）是全世界，特别是发展中国家的主要死亡原因。低密度脂蛋白（LDL）是CVD早期检测和干预的重要生物标志物，为CVD的诊断和预后提供了有价值的信息。在此，我们基于还原石墨烯oxide@molybdenum二硫化二茂铁羧酸（rGO@MoS2-Fc）组成的过氧化物酶类纳米酶开发了一种用于LDL检测的双信号电化学感应传感器。在电化学测试过程中，rGO@MoS2-Fc实现了电化学信号的协同放大，通过差分脉冲伏安法（DPV）检测Fc氧化电流增强，通过电流-时间（i-t）分析实现过氧化氢（H2O2）分解电流增加。与Fc氧化反应相对应的DPV信号在0.001 ~ 100.0 μg/mL范围内呈对数线性响应，检出限低至0.91 ng/mL。H2O2分解产生的i-t信号线性范围为1.0 ~ 80.0 μg/mL， LOD为0.80 μg/mL。该双信号策略具有良好的选择性、重复性、稳定性和自校准能力。对人血清样本的分析表明，该策略有望成为早期CVD诊断和临床管理的有力方法。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.

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