Silence of circPLK1 inhibits NSCLC progression by regulating the miR-1294/SLC16A9 axis

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular immunology Pub Date : 2026-01-01 Epub Date: 2025-12-23 DOI:10.1016/j.molimm.2025.11.020

Chuankui Li, Yifan Yang, Qicai Li, Guowen Wang, Tao Tao, Lixiang Li, Haiwei Sang

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引用次数: 0

Abstract

Background

Non-small cell lung cancer (NSCLC) is the malignancy with the highest mortality worldwide. Circular RNA polo-like kinase 1 (circPLK1) is a circular RNA that involved in cancer progression. However, the role of circPLK1 in NSCLC requires further investigation.

Methods

The expression of circPLK1 in NSCLC cells was measured by using qRT-PCR. Bioinformatics analysis was performed to identify miRNAs that bind to circPLK1 and the target mRNAs of these miRNAs. Cell viability, proliferation, apoptosis, migration, and invasion were assessed using CCK-8, colony formation, flow cytometry, and Transwell assays, respectively. Protein expression was evaluated by western blotting. Xenograft models were established to verify the regulatory role of circPLK1 in the miR-1294/SLC16A9 axis.

Results

circPLK1 was upregulaterd in NSCLC cells. Silencing circPLK1 markedly inhibited cell viability, proliferation, migration, and invasion, while accelerating apoptosis in NSCLC cells. circPLK1 acted as a sponge for miR-1294 and promoted SLC16A9 expression in NSCLC cells. Knockdown of circPLK1 suppressed tumor growth in NSCLC by regulating the miR-1294/SLC16A9 axis.

Conclusion

circPLK1 silence inhibited the proliferation, migration, and invasion of NSCLC cells by regulating the miR-1294/SLC16A9 axis.

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circPLK1沉默通过调节miR-1294/SLC16A9轴抑制NSCLC进展。

背景：非小细胞肺癌（NSCLC）是世界上死亡率最高的恶性肿瘤。环状RNA polo样激酶1 （circPLK1）是一种参与癌症进展的环状RNA。然而，circPLK1在非小细胞肺癌中的作用有待进一步研究。方法：采用qRT-PCR方法检测circPLK1在非小细胞肺癌细胞中的表达。进行生物信息学分析以鉴定与circPLK1结合的mirna和这些mirna的靶mrna。分别采用CCK-8、集落形成、流式细胞术和Transwell法评估细胞活力、增殖、凋亡、迁移和侵袭。western blotting检测蛋白表达。建立异种移植模型来验证circPLK1在miR-1294/SLC16A9轴中的调节作用。结果：circPLK1在NSCLC细胞中表达上调。沉默circPLK1可显著抑制非小细胞肺癌细胞的活力、增殖、迁移和侵袭，同时加速细胞凋亡。circPLK1作为miR-1294的海绵，促进SLC16A9在NSCLC细胞中的表达。敲低circPLK1通过调节miR-1294/SLC16A9轴抑制非小细胞肺癌的肿瘤生长。结论：circPLK1沉默通过调节miR-1294/SLC16A9轴抑制NSCLC细胞的增殖、迁移和侵袭。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular immunology 医学-免疫学

CiteScore

6.90

自引率

2.80%

发文量

324

审稿时长

50 days

期刊介绍： Molecular Immunology publishes original articles, reviews and commentaries on all areas of immunology, with a particular focus on description of cellular, biochemical or genetic mechanisms underlying immunological phenomena. Studies on all model organisms, from invertebrates to humans, are suitable. Examples include, but are not restricted to: Infection, autoimmunity, transplantation, immunodeficiencies, inflammation and tumor immunology Mechanisms of induction, regulation and termination of innate and adaptive immunity Intercellular communication, cooperation and regulation Intracellular mechanisms of immunity (endocytosis, protein trafficking, pathogen recognition, antigen presentation, etc) Mechanisms of action of the cells and molecules of the immune system Structural analysis Development of the immune system Comparative immunology and evolution of the immune system "Omics" studies and bioinformatics Vaccines, biotechnology and therapeutic manipulation of the immune system (therapeutic antibodies, cytokines, cellular therapies, etc) Technical developments.