Detection and genome characterization of beet mosaic virus naturally infecting fodder beet crop in Argentina

IF 2.5 2区农林科学 Q1 AGRONOMY

Crop Protection Pub Date : 2026-04-01 Epub Date: 2025-12-23 DOI:10.1016/j.cropro.2025.107529

Luciani Cecilia Elizabeth , Celli Marcos Giovani , Brugo Carivali María Florencia , Bongiorno Vanina Aylen , Conci Luis Rogelio , Fernandez Franco Daniel , Perotto María Cecilia

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引用次数: 0

Abstract

Fodder beet (Beta vulgaris subsp. vulgaris) is cultivated due its tolerance to low temperatures and poor soils as it improves the structure of forage production systems. Viral diseases represent a significant threat to beet production. Beet mosaic virus (BtMV) is the only confirmed potyvirus infecting beets. Between 2023 and 2024, plants showing virus-like symptoms from Argentinian main production area were surveyed. Transmission electron microscopy revealed flexuous filamentous particles consistent with BtMV. Oxford Nanopore Technologies sequencing of one sample yielded the complete BtMV genome (B12AR; 9451 nt, excluding the poly (A) tail), sharing 97.47 % amino acid identity with the BtMV-Wa isolate from the USA. Additionally, sequences of Beet cryptic virus 2, a Deltapartitivirus, were detected. RT-PCR confirmed BtMV infection in four of seven samples collected. These results would indicate the presence of other viruses not identified in this study. Phylogenetic analysis grouped B12AR within a clade with isolates from USA and China, distinct from a divergent lineage infecting wild beets. A recombination event involving P1 and HC-Pro regions, with parental sequences from the USA and South Africa was detected. Further studies are necessary to determine the etiology of fodder beet symptoms. This is the first report and complete genome sequence of BtMV infecting fodder beet in Argentina.

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阿根廷饲料用甜菜天然感染甜菜花叶病毒的检测及基因组特征分析

饲料用甜菜（Beta vulgaris subsp）由于其对低温和贫瘠土壤的耐受性，它改善了饲料生产系统的结构，因此被种植。病毒性疾病对甜菜生产构成重大威胁。甜菜花叶病毒（BtMV）是唯一确认的感染甜菜的马铃薯病毒。在2023年至2024年期间，对阿根廷主产区出现病毒样症状的植物进行了调查。透射电镜显示与BtMV一致的弯曲丝状颗粒。牛津纳米孔技术公司（Oxford Nanopore Technologies）对一个样本进行测序，获得了完整的BtMV基因组（B12AR; 9451 nt，不包括聚(A)尾），与美国BtMV- wa分离物具有97.47%的氨基酸同源性。此外，还检测到甜菜隐病毒2（一种三角病毒）的序列。在收集的7份样本中，有4份经RT-PCR证实感染了BtMV。这些结果表明存在本研究未发现的其他病毒。系统发育分析将B12AR与来自美国和中国的分离株归为一个分支，不同于感染野生甜菜的不同谱系。检测到涉及P1和HC-Pro区域的重组事件，亲本序列来自美国和南非。需要进一步的研究来确定饲料用甜菜症状的病因。这是阿根廷首次报道的BtMV感染饲料用甜菜的全基因组序列。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Protection 农林科学-农艺学

CiteScore

6.10

自引率

3.60%

发文量

200

审稿时长

29 days

期刊介绍： The Editors of Crop Protection especially welcome papers describing an interdisciplinary approach showing how different control strategies can be integrated into practical pest management programs, covering high and low input agricultural systems worldwide. Crop Protection particularly emphasizes the practical aspects of control in the field and for protected crops, and includes work which may lead in the near future to more effective control. The journal does not duplicate the many existing excellent biological science journals, which deal mainly with the more fundamental aspects of plant pathology, applied zoology and weed science. Crop Protection covers all practical aspects of pest, disease and weed control, including the following topics: -Abiotic damage- Agronomic control methods- Assessment of pest and disease damage- Molecular methods for the detection and assessment of pests and diseases- Biological control- Biorational pesticides- Control of animal pests of world crops- Control of diseases of crop plants caused by microorganisms- Control of weeds and integrated management- Economic considerations- Effects of plant growth regulators- Environmental benefits of reduced pesticide use- Environmental effects of pesticides- Epidemiology of pests and diseases in relation to control- GM Crops, and genetic engineering applications- Importance and control of postharvest crop losses- Integrated control- Interrelationships and compatibility among different control strategies- Invasive species as they relate to implications for crop protection- Pesticide application methods- Pest management- Phytobiomes for pest and disease control- Resistance management- Sampling and monitoring schemes for diseases, nematodes, pests and weeds.