Application value of TPAS staining technique for cervical exfoliated cells in cervical cancer screening.

IF 3.5 3区医学 Q2 PATHOLOGY

Diagnostic Pathology Pub Date : 2025-12-22 DOI:10.1186/s13000-025-01744-w

Guo Chen, Wenli Zhang, Yuchen HuYan, Yipching Yang, Wensheng Li, Guoqiang Feng, Zifan Lu

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引用次数: 0

Abstract

Background: Recent studies have shown that tumor cell membranes exhibit lower polarity than normal cell membranes, a characteristic that can be harnessed for cancer diagnosis. TPAS (viscosity-responsive plasma membrane probe), a recently developed staining method using cell membrane polarity probes, may selectively visualize cervical cancer cells by targeting membrane polarity differences, offering a potential new approach for cervical cancer screening.To investigate the diagnostic value of TPAS staining combined with liquid-based thin-layer cytological testing (TCT) and human papillomavirus (HPV) testing in detecting cervical cancer.

Methods: A total of 100 patients with suspected cervical precancerous lesions from the People's Hospital of Shaanxi Province between May 2024 and May 2025 were studied. All patients underwent TPAS testing, HPV testing and TCT. Biopsy results were the gold standard for evaluating positivity rates and diagnostic values of the tests, both individually and in combination.

Results: Among the 100 participants, the positive rates of the tests were as follows: TPAS detection rate was 86.7%, HPV detection rate was 80%, TCT rate 66.7%, and TCT + HPV rate was 35.00%. The combined TPAS + HPV testing showed higher accuracy (72.00%) and sensitivity(70.6%) than TCT + HPV (58.0%)and (54.1%), and the differences were statistically significant ((χ2 = 14.00,P = 0.0002).

Conclusion: TPAS combined with HPV testing has high specificity, sensitivity and accuracy, making it a promising approach for cervical cancer diagnosis.

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TPAS染色技术在宫颈癌筛查中的应用价值。

背景：最近的研究表明，肿瘤细胞膜表现出比正常细胞膜更低的极性，这一特征可以用于癌症诊断。TPAS （viscosity-responsive plasma membrane probe）是最近发展起来的一种利用细胞膜极性探针进行染色的方法，它可以通过靶向细胞膜极性差异来选择性地观察宫颈癌细胞，为宫颈癌筛查提供了潜在的新途径。探讨TPAS染色联合液基薄层细胞学检测（TCT）和人乳头瘤病毒（HPV）检测对宫颈癌的诊断价值。方法：选取2024年5月~ 2025年5月陕西省人民医院收治的100例疑似宫颈癌前病变患者为研究对象。所有患者均接受TPAS检测、HPV检测和TCT检测。活检结果是评估检测阳性率和诊断价值的金标准，无论是单独的还是联合的。结果：100例受试者中，TPAS检出率为86.7%，HPV检出率为80%，TCT检出率为66.7%，TCT + HPV检出率为35.00%。TPAS + HPV联合检测的准确率（72.00%）和灵敏度（70.6%）高于TCT + HPV（58.0%）和（54.1%），差异均有统计学意义（χ2 = 14.00,P = 0.0002）。结论：TPAS联合HPV检测具有较高的特异性、敏感性和准确性，是一种很有前景的宫颈癌诊断方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic Pathology 医学-病理学

CiteScore

4.60

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Diagnostic Pathology is an open access, peer-reviewed, online journal that considers research in surgical and clinical pathology, immunology, and biology, with a special focus on cutting-edge approaches in diagnostic pathology and tissue-based therapy. The journal covers all aspects of surgical pathology, including classic diagnostic pathology, prognosis-related diagnosis (tumor stages, prognosis markers, such as MIB-percentage, hormone receptors, etc.), and therapy-related findings. The journal also focuses on the technological aspects of pathology, including molecular biology techniques, morphometry aspects (stereology, DNA analysis, syntactic structure analysis), communication aspects (telecommunication, virtual microscopy, virtual pathology institutions, etc.), and electronic education and quality assurance (for example interactive publication, on-line references with automated updating, etc.).