Tumor-infiltrating neutrophil is an independent prognostic factor in pancreatic ductal adenocarcinoma.

IF 3.5 3区医学 Q2 PATHOLOGY

Diagnostic Pathology Pub Date : 2025-12-22 DOI:10.1186/s13000-025-01743-x

Seung-Myoung Son, Hye Sook Han, Ho-Chang Lee, Ok-Jun Lee, Chang Gok Woo

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引用次数: 0

Abstract

Background: Pancreatic ductal adenocarcinoma (PDAC) remains one of the most lethal malignancies. Tumor-infiltrating neutrophils (TINs) have been implicated in tumor progression and poor prognosis; however, their roles in PDAC remain unclear. TINs have been shown to granulocytic myeloid-derived suppressor cells (G-MDSCs) which inhibit adaptive immune responses and share morphological and functional features with classical neutrophils. G-MDSCs, unlike classical neutrophils, express myeloperoxidase (MPO). We evaluated the significance of TINs and MPO expression as a marker for prognosis in the PDAC.

Methods: We retrospectively analyzed 128 patients with surgically resected PDAC. The presence of TIN was assessed on hematoxylin and eosin-stained slides and immunohistochemistry was performed for MPO.

Results: TINs were identified in 61.7% of patients with PDAC. Their presence was significantly associated with worse overall survival and recurrence-free survival. After adjusting for clinicopathological variables, TIN remained an independent predictor of a poor prognosis. MPO was expressed in all TIN within PDAC, but was absent in neutrophils within benign inflammatory conditions.

Conclusions: TIN in PDAC represents an independent adverse prognostic factor and is likely to be G-MDSCs based on MPO expression. Assessing TIN and MPO status in preoperative biopsy specimens may offer valuable prognostic information and guide future therapeutic strategies for PDAC.

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肿瘤浸润性中性粒细胞是胰腺导管腺癌的独立预后因素。

背景：胰腺导管腺癌（PDAC）仍然是最致命的恶性肿瘤之一。肿瘤浸润中性粒细胞（TINs）与肿瘤进展和不良预后有关；然而，它们在PDAC中的作用尚不清楚。研究表明，TINs对粒细胞髓源性抑制细胞（G-MDSCs）具有抑制适应性免疫反应的作用，与经典中性粒细胞具有相同的形态和功能特征。与传统的中性粒细胞不同，G-MDSCs表达髓过氧化物酶（MPO）。我们评估了tin和MPO表达作为PDAC预后指标的意义。方法：回顾性分析128例手术切除的PDAC患者。在苏木精和伊红染色玻片上评估TIN的存在，并对MPO进行免疫组化。结果：61.7%的PDAC患者发现了tin。它们的存在与较差的总生存期和无复发生存期显著相关。在调整临床病理变量后，TIN仍然是预后不良的独立预测因子。MPO在PDAC的所有TIN中表达，但在良性炎症条件下中性粒细胞中不表达。结论：TIN在PDAC中是一个独立的不良预后因素，根据MPO的表达，可能是G-MDSCs。评估术前活检标本中的TIN和MPO状态可能提供有价值的预后信息，并指导PDAC的未来治疗策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic Pathology 医学-病理学

CiteScore

4.60

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Diagnostic Pathology is an open access, peer-reviewed, online journal that considers research in surgical and clinical pathology, immunology, and biology, with a special focus on cutting-edge approaches in diagnostic pathology and tissue-based therapy. The journal covers all aspects of surgical pathology, including classic diagnostic pathology, prognosis-related diagnosis (tumor stages, prognosis markers, such as MIB-percentage, hormone receptors, etc.), and therapy-related findings. The journal also focuses on the technological aspects of pathology, including molecular biology techniques, morphometry aspects (stereology, DNA analysis, syntactic structure analysis), communication aspects (telecommunication, virtual microscopy, virtual pathology institutions, etc.), and electronic education and quality assurance (for example interactive publication, on-line references with automated updating, etc.).