Multifunctional gelatin/phycocyanin composite hydrogel for infected wound therapy: pH-responsive drug release, antibacterial/antioxidant properties, and enhanced healing

IF 8.7 1区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

International Journal of Biological Macromolecules Pub Date : 2026-01-01 Epub Date: 2025-12-20 DOI:10.1016/j.ijbiomac.2025.149818

Yongduo Du , Yan Nie , Ran Zhang , Guokai Liu , Chunguang Ren , Wenjun Li , Zhihong Tang

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引用次数: 0

Abstract

Infected wound healing remains a critical challenge in clinical practice, necessitating advanced therapeutic strategies. This study developed multifunctional gelatin/phycocyanin (Gel/PC) composite hydrogels (GP) via EDC/NHS-activated amidation and THPS-mediated Mannich reaction. By systematically varying Gel, THPS, and PC concentrations, an optimal formulation (GP1: 20 % Gel, 0.4 % THPS, 1 % PC) was identified. GP1 exhibited a uniform porous microstructure, rapid gelation (~650 s), pH-responsive swelling, superior mechanical strength (fracture energy ~6.03 MJ/m³), excellent self-healing capacity, and strong tissue adhesion. The hydrogel demonstrated significant antioxidant activity (>80 % DPPH scavenging) and potent antibacterial effects against S. aureus (99.09 % inhibition) and E. coli (97.32 % inhibition). Loaded with polydeoxyribonucleotide (PDRN), the GPP hydrogel showed pH-responsive release with near-complete drug delivery (99.9 %) at wound pH (5.5). In a rat full-thickness wound model, GPP treatment achieved 81.34 % wound closure within 10 days, promoted re-epithelialization, enhanced collagen deposition (71.23 % area), attenuated inflammation (reduced IL-6 and TNF-α), and stimulated angiogenesis (increased VEGF). These results indicate that the GP hydrogel is a promising therapeutic platform for infected wounds.

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用于感染伤口治疗的多功能明胶/藻蓝蛋白复合水凝胶：ph反应性药物释放，抗菌/抗氧化特性，并促进愈合。

感染伤口愈合仍然是临床实践中的一个关键挑战，需要先进的治疗策略。本研究通过EDC/ nhs活化酰胺和thps介导的Mannich反应制备了多功能明胶/藻蓝蛋白（凝胶/PC）复合水凝胶（GP）。通过系统地改变凝胶，THPS和PC的浓度，确定了最佳配方（GP1: 20 %凝胶，0.4 % THPS, 1 % PC）。GP1具有均匀的孔隙结构、快速凝胶化（~650 s）、ph响应性膨胀、优异的机械强度（断裂能~6.03 MJ/m3）、优异的自愈能力和较强的组织粘附性。该水凝胶具有显著的抗氧化活性（bbb80 % DPPH清除率），对金黄色葡萄球菌（99.09 %抑制）和大肠杆菌（97.32 %抑制）具有较强的抗菌作用。GPP水凝胶负载多脱氧核糖核苷酸（PDRN），在伤口pH（5.5）下显示出pH响应性释放，几乎完全释放药物（99.9 %）。在大鼠全层创面模型中，GPP处理在10 天内实现81.34% %的创面愈合，促进再上皮化，增强胶原沉积（71.23% %的面积），减轻炎症（减少IL-6和TNF-α），刺激血管生成（增加VEGF）。这些结果表明GP水凝胶是一种很有前途的治疗感染伤口的平台。

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来源期刊

International Journal of Biological Macromolecules 生物-生化与分子生物学

CiteScore

13.70

自引率

9.80%

发文量

2728

审稿时长

64 days

期刊介绍： The International Journal of Biological Macromolecules is a well-established international journal dedicated to research on the chemical and biological aspects of natural macromolecules. Focusing on proteins, macromolecular carbohydrates, glycoproteins, proteoglycans, lignins, biological poly-acids, and nucleic acids, the journal presents the latest findings in molecular structure, properties, biological activities, interactions, modifications, and functional properties. Papers must offer new and novel insights, encompassing related model systems, structural conformational studies, theoretical developments, and analytical techniques. Each paper is required to primarily focus on at least one named biological macromolecule, reflected in the title, abstract, and text.