The Added Value of Brilliance GBS Agar for Screening Bulk Tank Milk for Streptococcus agalactiae

IF 3.7 4区农林科学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of food protection Pub Date : 2026-01-01 Epub Date: 2025-12-18 DOI:10.1016/j.jfp.2025.100685

Annet E. Heuvelink , Alessandro Bellato , Manon Holstege , Theo J.G.M. Lam

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引用次数: 0

Abstract

This study aimed to evaluate the added value of chromogenic Brilliance^TM GBS Agar to the generally used modified Edwards medium for the isolation of S. agalactiae from bovine bulk tank milk (BTM) samples in programs to monitor udder health and hygiene in dairy farms. During 10 BTM sampling rounds in a one-year study period, BTM samples were examined for the presence of S. agalactiae using both, modified Edwards medium and Brilliance^TM GBS Agar. The prevalence of S. agalactiae per sampling round based on modified Edwards medium alone varied from 0.7% to 2.1%, and based on the combination of modified Edwards medium and Brilliance^TM GBS Agar from 2.3% to 2.8% of samples. Of 1,352 herds with culture results available for all 10 sampling rounds, 59 (4.4%) tested positive for S. agalactiae at least once; 79.7% of these 59 herds were identified on modified Edwards medium and 93.2% on Brilliance^TM GBS Agar. Using both agars, the percentage of herds with at least one negative culture result between positive results decreased from 68.1% to 53.2% of positive herds, thus leading to a more consistent herd-level S. agalactiae diagnosis. However, for 10.6% of the herds, an irregular S. agalactiae culture result was introduced by using both agars. The use of Brilliance^TM GBS Agar makes the work of technicians easier and quicker in comparison with modified Edwards medium. For optimal sensitivity of S. agalactiae and of other streptococci(−like) bacteria, the combination of the two agars is needed.

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华晨GBS琼脂在散装罐乳中筛选无乳链球菌的附加价值。

本研究旨在评价显色型BrillianceTM GBS琼脂在牛乳样品中分离无乳链球菌的附加价值，以监测奶牛的乳房健康和卫生。在为期一年的研究期间，在10轮BTM取样中，使用改良Edwards培养基和BrillianceTM GBS琼脂检测BTM样品中有无无乳链球菌。单独使用改良Edwards培养基时，每轮取样无乳链球菌的流行率为0.7% ~ 2.1%，而使用改良Edwards培养基和BrillianceTM GBS琼脂的组合取样时，每轮取样无乳链球菌的流行率为2.3% ~ 2.8%。在所有10轮取样均获得培养结果的1352头畜群中，59头（4.4%）至少一次检测出无乳链球菌阳性；其中79.7%在改良Edwards培养基上鉴定，93.2%在BrillianceTM GBS琼脂上鉴定。使用这两种琼脂，在阳性结果之间至少有一个阴性培养结果的畜群百分比从阳性畜群的68.1%下降到53.2%，从而导致更一致的畜群水平无乳链球菌诊断。然而，10.6%的畜群在两种琼脂同时使用的情况下无乳链球菌培养结果不规则。与改良的爱德华兹培养基相比，使用BrillianceTM GBS琼脂使技术人员的工作更容易，更快。为了获得最佳的无乳链球菌和其他链球菌（样）细菌的敏感性，需要两种琼脂的组合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of food protection 工程技术-生物工程与应用微生物

CiteScore

4.20

自引率

5.00%

发文量

296

审稿时长

2.5 months

期刊介绍： The Journal of Food Protection® (JFP) is an international, monthly scientific journal in the English language published by the International Association for Food Protection (IAFP). JFP publishes research and review articles on all aspects of food protection and safety. Major emphases of JFP are placed on studies dealing with: Tracking, detecting (including traditional, molecular, and real-time), inactivating, and controlling food-related hazards, including microorganisms (including antibiotic resistance), microbial (mycotoxins, seafood toxins) and non-microbial toxins (heavy metals, pesticides, veterinary drug residues, migrants from food packaging, and processing contaminants), allergens and pests (insects, rodents) in human food, pet food and animal feed throughout the food chain; Microbiological food quality and traditional/novel methods to assay microbiological food quality; Prevention of food-related hazards and food spoilage through food preservatives and thermal/non-thermal processes, including process validation; Food fermentations and food-related probiotics; Safe food handling practices during pre-harvest, harvest, post-harvest, distribution and consumption, including food safety education for retailers, foodservice, and consumers; Risk assessments for food-related hazards; Economic impact of food-related hazards, foodborne illness, food loss, food spoilage, and adulterated foods; Food fraud, food authentication, food defense, and foodborne disease outbreak investigations.