Genomic insights into a multidrug-resistant uropathogenic Escherichia coli ST131-H30 strain associated with a fatal healthcare-associated infection

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

Journal of global antimicrobial resistance Pub Date : 2026-01-01 Epub Date: 2025-12-15 DOI:10.1016/j.jgar.2025.12.008

Samara Sant’Anna de Oliveira , Camille Alves Brito de Moura , Kaylanne Montenegro , Thereza Cristina da Costa Vianna , Ana Paula Alves do Nascimento , Gerson Gatto de Azeredo Coutinho , Hosana Dau Ferreira de Souza , Claudia Gladys Flores Sejas , Alexander Machado Cardoso , Kayo Bianco , Maysa Mandetta Clementino

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引用次数: 0

Abstract

Objective

Uropathogenic Escherichia coli (UPEC) poses a significant public health challenge due to increasing antimicrobial resistance (AMR) and severe infections. This study aimed to investigate the genetic heterogeneity and AMR mechanisms of a clinical UPEC strain associated with a fatal healthcare-associated infection.

Methods

Whole-genome sequencing (WGS) was performed on a multidrug-resistant (MDR) UPEC strain isolated from a pediatric patient by using an Illumina Miseq platform. The genome was assembled using Unicycler v0.4.8 and was annotated using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP). Genomic analysis included characterisation of the resistome, virulome, and phylogenetic profile using various bioinformatics tools such as ResFinder v4.7.2, VFDB, and PubMLST.

Results

The isolate has a genome with a size of 4,681,240 bp, a GC content of 50.60% and harbour seven plasmids. Genomic analysis revealed multiple genetic determinants contributing to a multidrug-resistant profile, including efflux systems, enzymatic target alterations, and antibiotic transporter modifications. Adhesin genes (fimH, afaA-D, lpfA, fdeC, csgA), hemolysins (hlyE, hlyF), iron acquisition systems (iucC, iutA, sitA), and genes related to antimicrobial peptide degradation (ompT, traJ) and colicin production (cma, cvaC) were identified. Resistance to heavy metals (tellurium, copper/silver, arsenic) was also detected. These findings characterise the isolate as a highly virulent, multidrug-resistant, and environmentally resilient emerging UPEC clone.

Conclusions

This study highlights the convergence of virulence, AMR, and environmental adaptability in an ST131-H30 UPEC strain. The findings underscore the critical need for comprehensive genomic surveillance, infection control strategies to mitigate the public health impact of MDR E. coli.

查看原文本刊更多论文

多药耐药尿路致病性大肠杆菌ST131-H30菌株与致命医疗保健相关感染的基因组分析

目的:。尿路致病性大肠杆菌（UPEC）由于抗菌素耐药性（AMR）和严重感染的增加而对公共卫生构成重大挑战。本研究旨在探讨与致命性医疗保健相关感染相关的临床UPEC菌株的遗传异质性和AMR机制。方法:。使用Illumina Miseq平台对从一名儿科患者分离的多药耐药（MDR） UPEC菌株进行全基因组测序（WGS）。使用Unicycler v0.4.8进行基因组组装，并使用NCBI原核基因组注释管道（PGAP）进行注释。基因组分析包括使用各种生物信息学工具（如ResFinder v4.7.2、VFDB和PubMLST）对抗性组、病毒组和系统发育谱进行表征。结果:。该分离物基因组大小为4,681,240 bp， GC含量为50.60%，含有7个质粒。基因组分析显示，多种遗传决定因素导致多药耐药，包括外排系统、酶靶改变和抗生素转运蛋白修饰。粘附素基因（fimH, afaA-D, lpfA, fdeC, csgA），溶血素基因（hye, hlyF），铁获取系统（iucC, iutA, sitA）以及抗菌肽降解相关基因（ompT, traJ）和粘菌素产生相关基因（cma, cvaC）被鉴定出来。对重金属（碲、铜/银、砷）的抗性也被检测到。这些发现将该分离物定性为高毒力、多药耐药和环境适应性强的新兴UPEC克隆。结论:。本研究强调了ST131-H30 UPEC菌株的毒力、抗菌素耐药性和环境适应性的趋同。这些发现强调了全面的基因组监测和感染控制战略的迫切需要，以减轻耐多药大肠杆菌对公共卫生的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

8.70

自引率

2.20%

发文量

285

审稿时长

34 weeks

期刊介绍： The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.