Kamal Bhattarai, Min Guo, Marya Bibi, Huayu Xu, Christian De Guzman, Xing-You Gu
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引用次数: 0
Abstract
Lack of seed dormancy (SD) often causes pre-harvest sprouting (PHS) or inadequate germination in cereal crops and seed production. This research aimed to integrate a dormancy (D) allele isolated from weedy rice at qSD12 into the genetic background of a restoration-of-fertility line (RFL) to mitigate the PHS and germination problems. A hybrid F2 population was developed to map quantitative trait loci (QTLs) and model their epistatic and genotype-by-environment interactions. Twelve QTLs for flowering time (FT, 3), plant height (5) or SD (4) were identified. Of the 4 SD loci, qSD12 explained most of the phenotypic variance (R2 = 0.34), and 2 (qSD3 & qSD6) have the D alleles from the RFL. qSD12 interacted with qSD3 or qSD7-2, with the presence of the D allele at qSD12 increasing the effects of the others, as well as the duration of seed storage at 24 or 40 ˚C for dormancy release. The F2 plant-derived F4 to F6 lines were marker-assisted selected. The major effect of qSD12 on seed germination and on-panicle sprouting was confirmed with the F4 and F5 lines, respectively. A sample of F2 plant-derived backcross (BC1F1) plants, which are heterozygous for 2-4 of the SD QTLs, was evaluated for agronomic traits and genotyped with an array of SNP markers. Recurrent backcrossing with the RFL and genomic selection are used to pyramid the D alleles from qSD12 and other locus/loci to develop new varieties and RF lines with improved resistance to PHS.
Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01623-8.
期刊介绍：
Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer.
All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others.
Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards.
Molecular Breeding core areas:
Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.
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