First identification of a multidrug-resistant Pseudomonas kurunegalensis clinical isolate co-carrying blaAFM-1 and blaIMP-1 in China

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

Journal of global antimicrobial resistance Pub Date : 2026-01-01 Epub Date: 2025-12-12 DOI:10.1016/j.jgar.2025.12.007

Shuwei Fan , Yuzhuo Gong , Chunxiao Chen , Jingchao Shi , Cong Wu

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引用次数: 0

Abstract

Objectives

The objective of this study was to elucidate the genetic context and characterize chromosome co-carrying bla_AFM-1 and bla_IMP-1 in a multidrug-resistant Pseudomonas kurunegalensis (P. kurunegalensis) isolate obtained from human urine using whole-genome sequencing.

Methods

This P. kurunegalensis isolate JC172 co-harbouring bla_AFM-1 and bla_IMP-1 underwent a comprehensive investigation that included antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing and bioinformatic analyses. To depict the evolutionary dynamics of P. kurunegalensis strains on a global scale, a phylogenetic tree was generated based on single nucleotide polymorphisms of core genomes.

Results

JC172 was classified as P. kurunegalensis based on sequencing results and average nucleotide identity (ANI) assessments. This isolate was designated as ST114 and possesses a chromosome with 5 855 340 base pairs, exhibiting a GC content of 61.9%. Several resistance genes, including bla_AFM-1 and bla_IMP-1, bla_OXA-246 and tmexC3D2-toprJ1 were identified on the chromosome. Genomic analysis revealed that bla_AFM-1 was situated within a core module of the TnAs3-bla_AFM-1 unit, which is bordered by class 1 integrons. This arrangement implies a significant potential for bla_AFM-1 dissemination.

Conclusions

Our study revealed that bla_AFM-1 and bla_IMP-1, bla_OXA-246 and tmexC3D2-toprJ1 were harboured by P. kurunegalensis. We suggested that ST114 P. kurunegalensis may serve as a reservoir for resistance genes.

查看原文本刊更多论文

中国首次发现一株多重耐药库鲁egalensis假单胞菌临床分离株共携带blaAFM-1和blaIMP-1。

目的：本研究的目的是利用全基因组测序技术，阐明从人类尿液中获得的多重耐药kurunegalensis假单胞菌（P. kurunegalensis）的遗传背景和染色体共携带blaAFM-1和blaIMP-1的特征。方法：对共携带blaAFM-1和blaIMP-1的kurunegalensis分离株JC172进行药敏试验、偶联试验、全基因组测序和生物信息学分析。为了描述p.k urunegalensis菌株在全球范围内的进化动态，基于核心基因组的单核苷酸多态性构建了系统发育树。结果：根据测序结果和平均核苷酸同源性（ANI）鉴定，JC172属kurunegalensis。该分离物被命名为ST114，具有一条5,855,340对碱基对的染色体，GC含量为61.9%。在染色体上鉴定出blaAFM-1和blaIMP-1、blaOXA-246和tmexc32d - toprj1等抗性基因。基因组分析显示，blaAFM-1位于TnAs3-blaAFM-1单元的核心模块中，该单元与1类整合子相邻。这种安排意味着blaAFM-1传播的巨大潜力。结论：我们的研究表明，blaAFM-1、blaIMP-1、blaOXA-246和tmexc32d - toprj1在库鲁尼加疟原虫中存在。我们认为ST114库鲁egalensis可能是抗性基因的储存库。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

8.70

自引率

2.20%

发文量

285

审稿时长

34 weeks

期刊介绍： The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.