R. N. Al Ebrahim, S. V. Bazhenov, M. Yu. Vyssokikh, Y. E. Liu, A. D. Vlasova, S. M. Bukhalovich, I. V. Manukhov
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引用次数: 0
Abstract
At present, biosensor tools for analyzing intracellular energy processes are actively being developed. In this work, a set of plasmid constructs with luciferase genes from Luciola mingrelica and Photorhabdus luminescens was proposed for obtaining biosensor cells based on bacteria and eukaryotes. The possibility of targeted expression of luciferase both in the cytoplasm and in the mitochondrial matrix was realized due to the use of signal sequences. It was demonstrated that the resulting biosensors are suitable for analyzing the cell pool of macromolecular compounds and reducing equivalents, such as ATP, NADH, and flavin mononucleotide (FMN). We hope that this tool will be in demand for the analysis of the activity of intracellular chaperones, as well as toxicological and other investigations.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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