Synergistic effects of magnesium-conditioned chitosan hydrogels and photobiomodulation on MC3T3-E1 preosteoblast function

IF 2.7 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy Pub Date : 2026-02-01 Epub Date: 2025-12-05 DOI:10.1016/j.pdpdt.2025.105307

Hao-Hsuan Chiu , Pei-Chun Wong , Ying-Chun Chen

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引用次数: 0

Abstract

Background

Magnesium (Mg) and photobiomodulation (PBM) have independently been reported to promote osteogenesis, but their combined effects remain unclear.

Methods

Mg-conditioned chitosan hygrogels (0,20, and 50 mol%) were prepared, and conditioned media were applied to MC3T3-E1 preosteoblasts with or without near-infrared PBM (830 nm, 5 J/cm²). Cell viability, migration, and calcium deposition were assessed (n = 3 biological replicates per group).

Results

High Mg reduced cell migration and mineralization, whereas PBM significantly enhanced calcium deposition and partially restored viability at early time points (p < 0.05). A notable synergistic effect was observed at 20 mol% Mg combined with PBM, particularly at specific time points, indicating a condition-dependent rather than uniformly optimal response.

Conclusion

The combination of Mg-conditioned hydrogels and PBM enhanced osteogenic responses in MC3T3-E1 preosteoblasts. This dual-modality strategy may inform scaffold design in bone tissue engineering.

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镁调壳聚糖水凝胶和光生物调节对MC3T3-E1成骨前细胞功能的协同作用。

背景：镁（Mg）和光生物调节（PBM）分别被报道促进骨生成，但它们的联合作用尚不清楚。方法：制备mg条件下的壳聚糖水凝胶（0、20、50 mol%），分别在近红外PBM （830 nm, 5 J/cm²）和不含PBM的MC3T3-E1成骨前细胞中应用条件培养基。评估细胞活力、迁移和钙沉积（每组n=3个生物重复）。结果：高Mg降低了细胞迁移和矿化，而PBM显著增强了钙沉积，在早期时间点部分恢复了细胞活力（p < 0.05）。在20 mol% Mg与PBM联合时，观察到显著的协同效应，特别是在特定的时间点，表明条件依赖而不是统一的最佳反应。结论：mg条件水凝胶与PBM联合作用可增强MC3T3-E1成骨前细胞的成骨反应。这种双模态策略可以为骨组织工程中的支架设计提供信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photodiagnosis and Photodynamic Therapy ONCOLOGY-

CiteScore

5.80

自引率

24.20%

发文量

509

审稿时长

50 days

期刊介绍： Photodiagnosis and Photodynamic Therapy is an international journal for the dissemination of scientific knowledge and clinical developments of Photodiagnosis and Photodynamic Therapy in all medical specialties. The journal publishes original articles, review articles, case presentations, "how-to-do-it" articles, Letters to the Editor, short communications and relevant images with short descriptions. All submitted material is subject to a strict peer-review process.