Cryogenically Structured Wide-Porous Gelatin–Hyaluronate Carrier of Peptide Bioregulator

Abstract

Using cryostructuring techniques, a new biocompatible, wide-porous gelatin-hyaluronate material was prepared, which was successfully tested as a carrier of a protein-peptide bioregulator. The following physicochemical parameters of the synthesized carrier were determined: the total water-holding capacity (51.2 ± 5.8 g H₂O/g polymer), the degree of swelling (7.03 ± 0.65 g H₂O/g polymer) of the polymer inside the macropore walls of the spongy material and the compressive modulus of elasticity (12.3 ± 1.5 kPa). The presence of a system of interconnected macropores with a cross section from 20 to 210 μm in the material is shown. Next, the resulting carrier was loaded with a protein-peptide bioregulator isolated from bovine sclera and used in the experiments on organ cultivation of a biological model: the posterior section of the newt eye. As a result, a significant protective effect of the scleral bioregulator included in the sponge carrier on the viability of tissues and cells of a biological model was revealed and the prospects of a new biocompatible cryogenically structured material for dosed release of the bioregulator into the culture medium was shown.