Performance comparison of rapid diagnostic test, microscopy, and polymerase chain reaction for Malaria detection in high-transmission area, Keerom Regency, Papua Province, Indonesia

IF 2.6 3区医学 Q2 PARASITOLOGY

Acta tropica Pub Date : 2026-01-01 Epub Date: 2025-12-05 DOI:10.1016/j.actatropica.2025.107935

Muhammad Fajri Rohkmad , Semuel Sandy , Mirna Widiyanti , Setyo Adiningsih , Agung Puja Kesuma , Yustinus Maladan , Yusnita Mirna Anggraeni , Tri Ramadhani , Mara Ipa , Muhammad Choirul Hidajat , Hanna Kawulur , Chandra Mukti Kurniawan , Adrianus Don Kopo , NLP Indi Dharmayanti , Triwibowo Ambar Garjito , Antonius Oktavian , Harimat Hendarwan

{"title":"Performance comparison of rapid diagnostic test, microscopy, and polymerase chain reaction for Malaria detection in high-transmission area, Keerom Regency, Papua Province, Indonesia","authors":"Muhammad Fajri Rohkmad , Semuel Sandy , Mirna Widiyanti , Setyo Adiningsih , Agung Puja Kesuma , Yustinus Maladan , Yusnita Mirna Anggraeni , Tri Ramadhani , Mara Ipa , Muhammad Choirul Hidajat , Hanna Kawulur , Chandra Mukti Kurniawan , Adrianus Don Kopo , NLP Indi Dharmayanti , Triwibowo Ambar Garjito , Antonius Oktavian , Harimat Hendarwan","doi":"10.1016/j.actatropica.2025.107935","DOIUrl":null,"url":null,"abstract":"<div><div>This study evaluates the performance of three diagnostic methods for malaria detection, Rapid Diagnostic Tests (RDTs), microscopy, and Polymerase Chain Reaction (PCR), in the high-transmission area of Keerom Regency, Papua Province, Indonesia. With malaria posing a significant health threat, particularly in Indonesia, accurate and timely diagnosis is crucial for effective treatment and disease management. The study involved 234 participants suspected of having uncomplicated malaria, with blood samples analyzed using all three diagnostic methods. Results indicated that PCR identified 42.3 % of malaria cases, while microscopy and RDTs detected 26.5 % and 22.6 %, respectively. Notably, PCR revealed a substantial number of cases missed by microscopy and RDTs, highlighting the limitations of these methods in low-parasitemia situations. The sensitivity and specificity of microscopy and RDTs were assessed against PCR as the reference standard, revealing significant discrepancies in diagnostic accuracy. The findings underscore the need for enhanced diagnostic strategies to improve case detection and support elimination efforts in Keerom regency, Papua, Indonesia</div></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":"273 ","pages":"Article 107935"},"PeriodicalIF":2.6000,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta tropica","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0001706X25004048","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/12/5 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

This study evaluates the performance of three diagnostic methods for malaria detection, Rapid Diagnostic Tests (RDTs), microscopy, and Polymerase Chain Reaction (PCR), in the high-transmission area of Keerom Regency, Papua Province, Indonesia. With malaria posing a significant health threat, particularly in Indonesia, accurate and timely diagnosis is crucial for effective treatment and disease management. The study involved 234 participants suspected of having uncomplicated malaria, with blood samples analyzed using all three diagnostic methods. Results indicated that PCR identified 42.3 % of malaria cases, while microscopy and RDTs detected 26.5 % and 22.6 %, respectively. Notably, PCR revealed a substantial number of cases missed by microscopy and RDTs, highlighting the limitations of these methods in low-parasitemia situations. The sensitivity and specificity of microscopy and RDTs were assessed against PCR as the reference standard, revealing significant discrepancies in diagnostic accuracy. The findings underscore the need for enhanced diagnostic strategies to improve case detection and support elimination efforts in Keerom regency, Papua, Indonesia

查看原文本刊更多论文

印度尼西亚巴布亚省Keerom县疟疾高传播区快速诊断检测、显微镜和聚合酶链反应的性能比较

本研究在印度尼西亚巴布亚省Keerom县的高传播地区评估了三种疟疾检测诊断方法的性能，即快速诊断试验（RDTs）、显微镜和聚合酶链反应（PCR）。由于疟疾对健康构成重大威胁，特别是在印度尼西亚，准确和及时的诊断对于有效治疗和疾病管理至关重要。这项研究涉及234名被怀疑患有非复杂性疟疾的参与者，他们的血液样本使用了所有三种诊断方法进行分析。结果表明，PCR检出42.3%的疟疾病例，显微镜和RDTs检出率分别为26.5%和22.6%。值得注意的是，PCR发现了大量显微镜和rdt未发现的病例，突出了这些方法在低寄生虫血症情况下的局限性。显微镜和rdt的敏感性和特异性与PCR作为参考标准进行了评估，发现诊断准确性存在显着差异。调查结果强调需要加强诊断战略，以改善印度尼西亚巴布亚Keerom县的病例发现和支持消除工作。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Acta tropica 医学-寄生虫学

CiteScore

5.40

自引率

11.10%

发文量

383

审稿时长

37 days

期刊介绍： Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.