Development of a colloidal gold-based immunochromatographic strip assay for rapid detection of Gyrovirus homsa 1 (GyH1)

IF 1.7 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2026-04-01 Epub Date: 2025-12-04 DOI:10.1016/j.jviromet.2025.115320

Qi Yang , Ziqiang Cheng , Tianxing Yan , Guihua Wang

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引用次数: 0

Abstract

Gyrovirus homsa 1 (GyH1) is an established causative agent of transmissible viral proventriculitis (TVP) in chickens. This study developed a colloidal gold immunochromatography assay (GICA) for GyH1 detection. Polyclonal and monoclonal antibodies against recombinant VP1 protein were generated. Colloidal gold nanoparticles were synthesized using the trisodium citrate reduction method, yielding uniform particle. Monoclonal antibodies were conjugated to colloidal gold particles, while polyclonal antibodies served as capture reagents immobilized on the membrane. Optimal labeling conditions were determined as pH 8.8 with a monoclonal concentration of 7.2 μg/mL. The assay demonstrated specific recognition of GyH1with a detection limit of 10³ copies/μL. Batch-to-batch reproducibility was confirmed across all test strip. In summary, the GyH1 GICA exhibits high specificity, sensitivity, and reproducibility, providing an effective and low-cost tool for monitoring GyH1 early-stage infection status and technical support for field detection applications.

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胶体金免疫层析条带法快速检测回旋病毒1型（GyH1）

涡旋病毒1 （GyH1）是鸡传染性病毒性脑室炎（TVP）的一种确定的病原体。本研究建立了一种检测GyH1的胶体金免疫层析法（GICA）。制备了重组VP1蛋白的多克隆和单克隆抗体。采用柠檬酸三钠还原法制备了胶体金纳米颗粒，得到了均匀的颗粒。单克隆抗体与胶体金结合，多克隆抗体作为固定在膜上的捕获试剂。最佳标记条件为pH 8.8，单克隆浓度为7.2 μg/mL。检测限为103拷贝/μL，对gyh11具有特异性识别。所有测试条都确认了批对批的重复性。综上所述，GyH1 GICA具有高特异性、敏感性和重复性，为监测GyH1早期感染状态提供了有效和低成本的工具，为现场检测应用提供了技术支持。

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.