Enhancing tomato plant micropropagation using copper oxide nanoparticles synthesized from copper complexes of (Z)-N’-(3-ethoxy-2-hydroxybenzylidene)benzohydrazide

IF 5.45 Q1 Physics and Astronomy

Nano-Structures & Nano-Objects Pub Date : 2026-02-01 Epub Date: 2025-12-04 DOI:10.1016/j.nanoso.2025.101584

M.S. Meenukutty, Arsha P. Mohan, V.G. Vidya (Professor), V.G. Viju Kumar

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引用次数: 0

Abstract

Copper oxide nanoparticles (CuO NPs) are emerging as promising tools in plant biotechnology, particularly for improving tissue culture techniques. However, their application in tomato micropropagation is still not well understood. This study explores the impact of CuO NPs on the in vitro regeneration of Solanum lycopersicum L. cv. Sankranti, aiming to determine their effectiveness in promoting plant growth and development under controlled conditions.CuO NPs were synthesized by calcining copper(II) complexes of (Z)-N’-(3-ethoxy-2-hydroxybenzylidene)benzohydrazide, prepared from different copper salts: CuSO₄·5H₂O, CuCl₂·2H₂O, and Cu(NO₃)₂·3H₂O. These complexes were characterized using FT-IR, UV-Vis spectroscopy, mass spectrometry, thermogravimetric analysis (TGA), magnetic studies, and molar conductance. The nanoparticles were further analysed using FT-IR, UV-Vis, PXRD, SEM, and TEM to confirm their structure and morphology. To evaluate their biological effect, CuO NPs were added to Murashige and Skoog (MS) medium at varying concentrations (0.02, 0.2, and 2.0 mg/L) and tested on tomato nodal explants. Statistical analysis using one-way ANOVA confirmed that these enhancements were significant (p < 0.05) when compared to the control group. Overall, this study highlights the potential of CuO NPs, particularly at optimized concentrations, to serve as beneficial micronutrient additives in plant tissue culture. These results support their future application in improving micropropagation efficiency for tomato and possibly other high-value crops.

查看原文本刊更多论文

以(Z)- n ' -（3-乙氧基-2-羟基苄基）苯并肼铜配合物合成氧化铜纳米颗粒促进番茄植株微繁殖

氧化铜纳米颗粒（CuO NPs）正在成为植物生物技术中有前景的工具，特别是在改善组织培养技术方面。然而，它们在番茄微繁中的应用还不是很清楚。本研究探讨了CuO NPs对番茄植株离体再生的影响。旨在确定它们在受控条件下促进植物生长发育的有效性。以不同的铜盐CuSO₄·5H₂O、CuCl₂·2H₂O和Cu（NO₃）₂·3H₂O为原料，通过煅烧(Z)- n′-（3-乙氧基-2-羟基苄基）苯并肼铜（II）配合物合成CuO NPs。这些配合物通过FT-IR、UV-Vis光谱、质谱、热重分析（TGA）、磁性研究和摩尔电导进行了表征。利用FT-IR、UV-Vis、PXRD、SEM、TEM等分析了纳米颗粒的结构和形貌。为评价CuO NPs的生物学效应，将不同浓度（0.02、0.2和2.0 mg/L）的CuO NPs分别添加到Murashige和Skoog （MS）培养基中，并在番茄外植体上进行试验。使用单因素方差分析的统计分析证实，与对照组相比，这些增强是显著的（p <； 0.05）。总的来说，本研究强调了CuO NPs的潜力，特别是在优化浓度下，作为植物组织培养中有益的微量营养素添加剂。这些结果支持了它们在提高番茄和其他高价值作物的微繁效率方面的未来应用。

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来源期刊

Nano-Structures & Nano-Objects Physics and Astronomy-Condensed Matter Physics

CiteScore

9.20

自引率

0.00%

发文量

审稿时长

22 days

期刊介绍： Nano-Structures & Nano-Objects is a new journal devoted to all aspects of the synthesis and the properties of this new flourishing domain. The journal is devoted to novel architectures at the nano-level with an emphasis on new synthesis and characterization methods. The journal is focused on the objects rather than on their applications. However, the research for new applications of original nano-structures & nano-objects in various fields such as nano-electronics, energy conversion, catalysis, drug delivery and nano-medicine is also welcome. The scope of Nano-Structures & Nano-Objects involves: -Metal and alloy nanoparticles with complex nanostructures such as shape control, core-shell and dumbells -Oxide nanoparticles and nanostructures, with complex oxide/metal, oxide/surface and oxide /organic interfaces -Inorganic semi-conducting nanoparticles (quantum dots) with an emphasis on new phases, structures, shapes and complexity -Nanostructures involving molecular inorganic species such as nanoparticles of coordination compounds, molecular magnets, spin transition nanoparticles etc. or organic nano-objects, in particular for molecular electronics -Nanostructured materials such as nano-MOFs and nano-zeolites -Hetero-junctions between molecules and nano-objects, between different nano-objects & nanostructures or between nano-objects & nanostructures and surfaces -Methods of characterization specific of the nano size or adapted for the nano size such as X-ray and neutron scattering, light scattering, NMR, Raman, Plasmonics, near field microscopies, various TEM and SEM techniques, magnetic studies, etc .