Dynamin 2 is involved in osteoblast migration by regulating the organization of F-actin

IF 2.2 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Oral Biosciences Pub Date : 2026-02-01 Epub Date: 2025-12-02 DOI:10.1016/j.job.2025.100720

Takumi Moriya , A. Surong , Nanami Tatsumi , Hiroshi Yamada , Fumiko Takemoto , Hiroshi Kamioka , Hirohiko Okamura , Mika Ikegame

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Abstract

Objectives

Dynamin, a GTPase that regulates membrane dynamics, has recently been implicated in actin cytoskeletal remodeling. This study aimed to elucidate the role of dynamin in osteoblast migration by examining the effects of dynamin inhibition on the localization and organization of F-actin and dynamin 2 in MC3T3-E1 cells.

Methods

MC3T3-E1 cells were treated with dynamin inhibitors (Dyngo 4a and Dynole 34-2), and cell migration was assessed using a wound-healing assay. Fluorescent staining was performed to analyze the intracellular localization of F-actin and dynamin 2.

Results

Dynamin inhibition significantly reduced the migration of MC3T3-E1 cells. Fluorescence analysis revealed a marked decrease in the accumulation and colocalization of F-actin and dynamin 2 at the protrusion edge. Additionally, dynamin inhibition suppressed the formation of lamellipodia and stress fibers while promoting the appearance of abnormal F-actin clusters in the cytoplasm.

Conclusions

These findings suggest that dynamin plays an essential role in osteoblast migration by regulating actin cytoskeletal remodeling, particularly through the formation of lamellipodia and stress fibers.

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动力蛋白2通过调节f -肌动蛋白的组织参与成骨细胞的迁移

目的动态蛋白是一种调节膜动力学的GTPase，最近被认为与肌动蛋白细胞骨架重塑有关。本研究旨在通过检测动力蛋白抑制对MC3T3-E1细胞F-actin和动力蛋白2的定位和组织的影响，阐明动力蛋白在成骨细胞迁移中的作用。方法用动力蛋白抑制剂（dyngo4a和Dynole 34-2）处理smc3t3 - e1细胞，采用伤口愈合实验评估细胞迁移。荧光染色分析F-actin和dynamin 2在细胞内的定位。结果dynamin抑制显著降低MC3T3-E1细胞的迁移能力。荧光分析显示，F-actin和dynamin 2在突起边缘的聚集和共定位明显减少。此外，动力蛋白抑制抑制板足和应激纤维的形成，同时促进细胞质中异常f -肌动蛋白簇的出现。结论动力蛋白通过调节肌动蛋白细胞骨架重塑，特别是通过板足和应力纤维的形成，在成骨细胞迁移中起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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