Integrative regulation of axillary meristem maturation and stolon fate determination in strawberry by light, gibberellin, and ZFP6

IF 24.1 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Plant Pub Date : 2025-12-05 DOI:10.1016/j.molp.2025.12.001

Lei Guo, Muzi Li, Xi Luo, Tianlong He, Ning Ma, Shaojun Tang, Zhongchi Liu

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Abstract

The strawberry axillary-meristem can develop into a branch crown (a flowering shoot) or a stolon (a horizontal stem that produces daughter plants), with gibberellin promoting stolon fate. Despite its importance for plant architecture, asexual reproduction, and perennial growth, the regulatory mechanism governing this fate decision remains poorly understood. We found that the juvenile-to-adult transition of the axillary-meristem is marked by induction of GA20ox4 expression and the onset of stolon formation. RNA-sequencing of staged meristems identified the zinc-finger protein ZFP6, which is strongly co-expressed with GA20ox4. CRISPR knockout of ZFP6 abolished GA20ox4 expression and eliminated stolon formation, a phenotype rescued by exogenous gibberellin, demonstrating that ZFP6 acts to activate gibberellin biosynthesis and promote stolon formation. Genetic analysis of mutants in the red-light receptor PhyB, together with mutants in gibberellin biosynthesis and signaling, further revealed that red light and PhyB promote axillary-meristem maturation-a previously unrecognized developmental stage that precedes fate determination. Together, these findings illuminate how developmental stage and environmental cues converge to regulate axillary-meristem maturation and fate determination and identify a stage-specific regulator controlling the switch to stolon formation.

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光、赤霉素和ZFP6对草莓腋生分生组织成熟和匍匐茎命运的综合调控

草莓的腋生分生组织可以发育成分枝冠（开花的枝条）或匍匐茎（产生子植株的水平茎），赤霉素促进匍匐茎的形成。尽管它对植物结构、无性生殖和多年生生长很重要，但控制这种命运决定的调节机制仍然知之甚少。我们发现腋窝分生组织从幼体到成体的转变是以诱导GA20ox4的表达和匍匐茎形成的开始为标志的。分生组织的rna测序鉴定出锌指蛋白ZFP6，该蛋白与GA20ox4强共表达。CRISPR敲除ZFP6后，GA20ox4表达被抑制，匍匐茎形成被消除，这是外源赤霉素拯救的表型，表明ZFP6激活了赤霉素的生物合成，促进了匍匐茎的形成。对红光受体PhyB突变体以及赤霉素生物合成和信号转导突变体的遗传分析进一步揭示，红光和PhyB促进腋窝分生组织成熟，这是一个在命运决定之前未被认识到的发育阶段。总之，这些发现阐明了发育阶段和环境因素如何共同调节腋窝分生组织的成熟和命运决定，并确定了一个控制向匍匐茎形成转换的阶段特异性调节因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Plant 植物科学-生化与分子生物学

CiteScore

37.60

自引率

2.20%

发文量

1784

审稿时长

1 months

期刊介绍： Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.