The role of miR-491-3p in early diagnosis and prognosis evaluation of acute myocardial infarction

IF 3.5 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Archives of biochemistry and biophysics Pub Date : 2026-02-01 Epub Date: 2025-12-03 DOI:10.1016/j.abb.2025.110691

Tai Dou , Lijie Qin , Peirong Zhang, Lijun Xu, Yanwei Cheng, Peng Wang

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引用次数: 0

Abstract

Aims

To investigate the expression characteristics, clinical implications, and underlying mechanisms of miR-491-3p in patients with acute myocardial infarction (AMI).

Methods

Serum samples were collected from patients with AMI and healthy controls. miR-491-3p expression was measured using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Receiver operating characteristic (ROC) curves evaluated its diagnostic accuracy for AMI. Correlations between miR-491-3p levels and myocardial injury markers or inflammatory factors were analyzed. A one-year follow-up assessed its predictive value for major adverse cardiovascular events (MACE). A hypoxia/reoxygenation (H/R) model of human AC16 cardiomyocytes was established to explore the mechanism of miR-491-3p in H/R-induced injury via targeting aquaporin 9 (AQP9).

Results

Serum miR-491-3p was significantly downregulated in AMI patients, with an AUC of 0.893 for AMI diagnosis (sensitivity 83 %, specificity 79.5 %). Levels were negatively correlated with myocardial injury markers and inflammatory factors. AMI patients with low miR-491-3p expression had a higher incidence of MACE, and low expression was identified as a risk factor. In the H/R model, miR-491-3p was downregulated. Overexpression of miR-491-3p improved cell proliferation, reduced apoptosis, and decreased inflammatory factors by targeting and inhibiting AQP9.

Conclusions

miR-491-3p may be involved in the pathological process of AMI by targeting and regulating AQP9. This makes it a promising candidate as both a diagnostic and prognostic marker for AMI, as well as a potential therapeutic target.

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miR-491-3p在急性心肌梗死早期诊断及预后评价中的作用

目的：探讨miR-491-3p在急性心肌梗死（AMI）患者中的表达特征、临床意义及其机制。方法：分别采集AMI患者和健康对照者的血清样本。采用逆转录定量聚合酶链反应（RT-qPCR）检测miR-491-3p的表达。受试者工作特征（ROC）曲线评价其诊断AMI的准确性。分析miR-491-3p水平与心肌损伤标志物或炎症因子的相关性。1年随访评估其对主要不良心血管事件（MACE）的预测价值。建立人AC16心肌细胞缺氧/再氧化（H/R）模型，通过靶向水通道蛋白9 （AQP9）探讨miR-491-3p在H/R诱导损伤中的作用机制。结果：AMI患者血清miR-491-3p明显下调，AMI诊断AUC为0.893（敏感性83%，特异性79.5%）。其水平与心肌损伤标志物和炎症因子呈负相关。miR-491-3p低表达的AMI患者MACE发生率较高，低表达被认为是一个危险因素。在H/R模型中，miR-491-3p下调。过表达miR-491-3p通过靶向和抑制AQP9促进细胞增殖，减少细胞凋亡，降低炎症因子。结论：miR-491-3p可能通过靶向调控AQP9参与AMI的病理过程。这使其成为AMI的诊断和预后标志物，以及潜在的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of biochemistry and biophysics 生物-生化与分子生物学

CiteScore

7.40

自引率

0.00%

发文量

245

审稿时长

26 days

期刊介绍： Archives of Biochemistry and Biophysics publishes quality original articles and reviews in the developing areas of biochemistry and biophysics. Research Areas Include: • Enzyme and protein structure, function, regulation. Folding, turnover, and post-translational processing • Biological oxidations, free radical reactions, redox signaling, oxygenases, P450 reactions • Signal transduction, receptors, membrane transport, intracellular signals. Cellular and integrated metabolism.