Thrombomodulin and CXCL6 Altered Expression in Preeclampsia: Serum-Placental Biomarker Correlations and Clinical Significance

IF 2.4 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology Pub Date : 2025-12-06 DOI:10.1111/aji.70192

Xiaobao Lai, Lihua Sheng, Zhihua Liao, Shan Wu, Mengqi Lin, Xin Zeng, Pan Huang, Weiming Yang, Juanjuan Chen

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引用次数: 0

Abstract

Objective

We aimed to examine the expression levels of thrombomodulin (THBD) and C-X-C motif ligand 6 (CXCL6/GCP-2) in the serum and placental tissues of pregnant women with preeclampsia (PE) and assess their clinical significance.

Methods

In this cross-sectional study, a cohort comprising 96 pregnant women diagnosed with preeclampsia (PE) and 119 healthy pregnant counterparts was selected from Jiangxi Maternal and Child Health Hospital, encompassing January 2024 to December 2024. The levels of THBD and CXCL6 in serum and placental tissues were assessed. The Mann‒Whitney U test was used to compare the differences between two groups, and the Spearman test was used to evaluate the risk factors for PE via correlation analysis.

Results

Serum THBD and CXCL6 levels were significantly elevated in the PE group compared to controls (p < 0.001), with a positive correlation between the two markers (r = 0.41, p < 0.001). Immunohistochemical analysis demonstrated reduced THBD and CXCL6 expression in placental syncytiotrophoblasts (p < 0.05), alongside diminished THBD immunoreactivity in vascular endothelial cells (p < 0.05). Multivariate logistic regression identified CXCL6, uric acid (UA), and lactate dehydrogenase (LDH) as independent risk factors for PE, while albumin (Alb) emerged as a protective factor (p < 0.05).

Conclusions

In pregnant women with PE, the serum levels of THBD and CXCL6 are increased, whereas their expression in placental tissue is decreased. CXCL6 emerges as an independent risk factor for PE, with expression patterns independent of gestational age confounding, while THBD shows PE-specific gestational age-related effects that provide additional insights into disease pathophysiology. These findings indicate that the inflammatory response and insufficient trophoblast invasion may play important roles in the pathogenesis of PE, providing a new theoretical basis for the early diagnosis and targeted intervention of PE.

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血栓调节素和CXCL6在子痫前期的表达改变：血清-胎盘生物标志物相关性及临床意义

目的探讨血栓调节素（THBD）和C-X-C基序配体6 （CXCL6/GCP-2）在孕妇子痫前期（PE）血清和胎盘组织中的表达水平及其临床意义。方法采用横断面研究方法，选取江西省妇幼保健院诊断为子痫前期（PE）的孕妇96例和健康孕妇119例，时间为2024年1月至2024年12月。测定血清和胎盘组织中THBD和CXCL6的水平。采用Mann-Whitney U检验比较两组间的差异，采用Spearman检验通过相关分析评价PE的危险因素。结果PE组血清THBD和CXCL6水平明显高于对照组（p < 0.001），两者呈正相关（r = 0.41, p < 0.001）。免疫组织化学分析显示胎盘合胞滋养细胞中THBD和CXCL6表达降低（p < 0.05），血管内皮细胞中THBD免疫反应性降低（p < 0.05）。多因素logistic回归发现CXCL6、尿酸（UA）和乳酸脱氢酶（LDH）是PE的独立危险因素，而白蛋白（Alb）是PE的保护因素（p < 0.05）。结论妊娠PE患者血清中THBD和CXCL6水平升高，而胎盘组织中THBD和CXCL6表达降低。CXCL6是PE的独立危险因素，其表达模式独立于胎龄混淆，而THBD显示PE特异性胎龄相关效应，为疾病病理生理学提供了额外的见解。提示炎症反应和滋养细胞侵袭不足可能在PE的发病机制中发挥重要作用，为PE的早期诊断和靶向干预提供了新的理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Reproductive Immunology 医学-免疫学

CiteScore

6.20

自引率

5.60%

发文量

314

审稿时长

2 months

期刊介绍： The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.