Guaiane-type sesquiterpenoids from patchouli oil and their potential anti-depressive effects

IF 3.4 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Phytochemistry Pub Date : 2026-03-01 Epub Date: 2025-12-03 DOI:10.1016/j.phytochem.2025.114738

Qi Zheng , Huan Zhu , He-ping Yang , Cheng Peng , Guang-xu Wu , Qin-mei Zhou , Liang Xiong

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Abstract

Sixteen guaiane-type sesquiterpenoids, including four undescribed 4,5-seco-guaiane derivatives (1–4) and one undescribed guaiane (6), were isolated from the volatile oil of Pogostemon cablin. Their structures were determined using extensive spectroscopic evidence, including NMR, IR, HR-ESI-MS, and ECD calculations. Their anti-depressive effects were evaluated in corticosterone (CORT)-induced PC12 cells in vitro. Compound 5 (7-epi-chabrolidione A) was previously reported by our group to exhibit neuroprotective properties. However, the present study further demonstrated that compounds 1–5 had protective effects against CORT-induced injury in PC12 cells. Hoechst 33258 staining, high-content screening, and western blot analysis revealed that compound 5 activated the CaMKII/CREB/BDNF signaling pathway, thereby reducing apoptosis in CORT-injured PC12 cells and inhibiting intracellular calcium levels. Furthermore, compound 5 effectively rescued reserpine-induced depression and cognitive impairment in zebrafish in vivo. Collectively, these findings indicate that 4,5-seco-guaiane sesquiterpenoids from Pogostemon cablin (especially compound 5) possess potential anti-depressive activity.

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广藿香精油中愈创蓝型倍半萜类化合物及其潜在的抗抑郁作用。

从广藿香挥发油中分离得到16个愈创烷型倍半萜类化合物，包括4个未描述的4,5-次愈创烷衍生物（1-4）和1个未描述的愈创烷(6)。它们的结构是通过广泛的光谱证据确定的，包括NMR， IR， HR-ESI-MS和ECD计算。在皮质酮（CORT）诱导的PC12细胞中评估其抗抑郁作用。化合物5 （7-epi-chabrolidione A）已被本课题组报道具有神经保护作用。然而，本研究进一步证明化合物1-5对cort诱导的PC12细胞损伤具有保护作用。Hoechst 33258染色、高含量筛选和western blot分析显示，化合物5激活CaMKII/CREB/BDNF信号通路，从而减少cort损伤的PC12细胞的凋亡，抑制细胞内钙水平。此外，化合物5在体内有效地挽救了利血平诱导的斑马鱼抑郁和认知障碍。综上所述，这些发现表明从广藿香中提取的4,5-邻愈创木烷倍半萜类化合物（特别是化合物5）具有潜在的抗抑郁活性。

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来源期刊

Phytochemistry 生物-植物科学

CiteScore

6.40

自引率

7.90%

发文量

443

审稿时长

39 days

期刊介绍： Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.

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