Evaluation of the levels of Fractalkine (CX3CL1), TNF-α, and TGF-β in the gingival crevicular fluid/tissue of patients with gingival overgrowth: a cross-sectional observational study.

IF 3 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Applied Oral Science Pub Date : 2025-11-28 eCollection Date: 2025-01-01 DOI:10.1590/1678-7757-2025-0304

Nazife Hamurcu, Ahu Uraz Çörekci, Rahşan Ilikçi Sağkan, Serpil Cula, Deniz Özbay Çetiner

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引用次数: 0

Abstract

Background: Fractalkine (CX3CL1) is expressed by various cells, contributing to the pathogenesis of diseases such as diabetes mellitus, vascular pathologies, and rheumatoid arthritis via immunological mechanisms. The CX3CL1-CX3CR1 axis regulates cellular responses such as proliferation and collagen production, which are implicated in gingival overgrowth (GO).

Objectives: This study aimed to assess the levels of CX3CL1, tumor necrosis factor-α (TNF-α), and transforming growth factor-beta (TGF-β) in both gingival crevicular fluid (GCF) and gingival tissues among patients with biofilm- and amlodipine-induced GO. Additionally, the potential relationship between these biomarkers and clinical periodontal parameters was evaluated.

Methods: The study included 17 participants with biofilm-induced GO (Group I), 18 participants with amlodipine-induced GO (Group A), and 10 systemically healthy participants without GO (Control). CX3CL1, TNF-α, and TGF-β levels in GCF samples were assessed using enzyme-linked immunosorbent assay (ELISA). Moreover, mRNA expression levels of CX3CL1, TNF-α, and TGF-β in tissue samples were determined by quantitative real-time PCR (qPCR).

Results: The total GCF CX3CL1 level was significantly higher in Group I and Group A compared to controls. However, tissue CX3CL1 and TNF-α levels were significantly higher in Group I than Group A (p<0.05). In Group A, total GCF CX3CL1 levels showed a positive correlation with the gingival index (GI) (r=0.644), bleeding on probing (BOP) (r=0.622), and GCF volume (r=0.720). A significant positive correlation was observed between tissue CX3CL1 and TNF-α levels (r=0.762) (p<0.05). In Group I, a significant correlation was observed between total GCF CX3CL1 and TNF-α, TGF-β, and GCF volume levels, respectively (r=0.865, r=0.845, r=0.651). A positive correlation (p<0.05) was also found between tissue CX3CL1 and TNF-α and TGF-β levels, respectively (r=0.689, r=0.903).

Conclusion: CX3CL1 may have a potential role in the development of GO-associated tissue fibrosis and its inflammatory mechanisms.

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评估牙龈过度生长患者龈沟液/组织中裂裂素（CX3CL1）、TNF-α和TGF-β水平：一项横断面观察研究

背景：Fractalkine （CX3CL1）在多种细胞中表达，通过免疫机制参与糖尿病、血管病变、类风湿关节炎等疾病的发病。CX3CL1-CX3CR1轴调节细胞反应，如增殖和胶原生成，这与牙龈过度生长（GO）有关。目的：本研究旨在评估生物膜和氨氯地平诱导氧化石墨烯患者龈沟液（GCF）和牙龈组织中CX3CL1、肿瘤坏死因子-α (TNF-α)和转化生长因子-β (TGF-β)的水平。此外，还评估了这些生物标志物与临床牙周参数之间的潜在关系。方法：本研究包括17例生物膜诱导氧化石墨烯（I组）、18例氨氯地平诱导氧化石墨烯（A组）和10例全身健康的非氧化石墨烯（对照组）。采用酶联免疫吸附法（ELISA）检测GCF样品中CX3CL1、TNF-α、TGF-β水平。采用实时荧光定量PCR （qPCR）检测组织样品中CX3CL1、TNF-α、TGF-β mRNA的表达水平。结果：ⅰ组和A组GCF CX3CL1总水平明显高于对照组。然而，I组的组织CX3CL1和TNF-α水平明显高于A组(p结论：CX3CL1可能在氧化石墨烯相关组织纤维化的发展及其炎症机制中具有潜在作用。

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来源期刊

Journal of Applied Oral Science 医学-牙科与口腔外科

CiteScore

4.80

自引率

3.70%

发文量

审稿时长

4-8 weeks

期刊介绍： The Journal of Applied Oral Science is committed in publishing the scientific and technologic advances achieved by the dental community, according to the quality indicators and peer reviewed material, with the objective of assuring its acceptability at the local, regional, national and international levels. The primary goal of The Journal of Applied Oral Science is to publish the outcomes of original investigations as well as invited case reports and invited reviews in the field of Dentistry and related areas.