Strontium implantation improving cell proliferation and adhesion on titanium surfaces through CaSR/AKT signaling pathway.

IF 1.8 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Dental materials journal Pub Date : 2025-11-29 DOI:10.4012/dmj.2024-330

Qingyong Chen, Bingkun Cheng, Qingling Gao, Lei Cheng, Jiahuan He, Yingqi Liu

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Abstract

Surface physical and chemical modifications can effectively enhance implant biocompatibility and soft tissue adhesion. In this study, titanium (Ti) plates were modified by magnetron sputtering to incorporate strontium (Sr), and the effects of Sr-doped Ti materials on fibroblast proliferation and adhesion were systematically evaluated. The results demonstrated that Sr-doped Ti significantly improved L929 fibroblast proliferation, cellular morphology during adhesion, and adhesion strength. Surface characterization by scanning electron microscopy, energy-dispersive X-ray spectroscopy, and atomic force microscopy confirmed the successful incorporation of Sr and revealed changes in surface morphology and potential. RNA sequencing and Western blot analyses showed that Sr enhanced fibroblast adhesion by activating the calcium-sensing receptor (CaSR)/PKC/AKT signaling pathway. Moreover, pharmacological inhibition of CaSR effectively reversed the Sr-induced promotion of fibroblast adhesion and AKT activation. These findings suggest that Sr-functionalized Ti implants can promote fibroblast adhesion and early soft tissue integration, offering a promising strategy to improve implant success rates and prevent peri-implant diseases.

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锶通过CaSR/AKT信号通路促进细胞在钛表面的增殖和粘附。

表面物理和化学修饰可有效提高种植体的生物相容性和软组织粘附性。本研究采用磁控溅射法对钛（Ti）板进行改性，加入锶（Sr），系统评价了锶掺杂钛材料对成纤维细胞增殖和粘附的影响。结果表明，sr掺杂Ti显著改善了L929成纤维细胞的增殖、粘附过程中的细胞形态和粘附强度。通过扫描电子显微镜、能量色散x射线能谱和原子力显微镜的表面表征证实了Sr的成功掺入，并揭示了表面形貌和电位的变化。RNA测序和Western blot分析显示，Sr通过激活钙敏感受体(CaSR)/PKC/AKT信号通路增强成纤维细胞粘附。此外，CaSR的药理抑制有效逆转了sr诱导的成纤维细胞粘附和AKT活化的促进。这些发现表明，锶功能化钛种植体可以促进成纤维细胞粘附和早期软组织整合，为提高种植体成功率和预防种植体周围疾病提供了一种有希望的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dental materials journal 医学-材料科学：生物材料

CiteScore

4.60

自引率

4.00%

发文量

102

审稿时长

3 months

期刊介绍： Dental Materials Journal is a peer review journal published by the Japanese Society for Dental Materials and Devises aiming to introduce the progress of the basic and applied sciences in dental materials and biomaterials. The dental materials-related clinical science and instrumental technologies are also within the scope of this journal. The materials dealt include synthetic polymers, ceramics, metals and tissue-derived biomaterials. Forefront dental materials and biomaterials used in developing filed, such as tissue engineering, bioengineering and artificial intelligence, are positively considered for the review as well. Recent acceptance rate of the submitted manuscript in the journal is around 30%.