Preparation, physicochemical properties, and immunomodulatory activity of glycoproteins from Syngnathoides biaculeatus

IF 5.8 3区化学 Q1 CHEMISTRY, MEDICINAL

Natural Products and Bioprospecting Pub Date : 2025-12-01 DOI:10.1007/s13659-025-00551-6

Xuewei Xia, Jieling Lin, Lihong Yang, Youhong Li, Wenshen Lin, Qingqing Wang, Jun Liu, Riming Huang

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Abstract

Syngnathoides biaculeatus, a potential functional food from marine sources, was found to enhance nonspecific immunity, but its functional ingredients have rarely been reported. Therefore, this study focused on the preparation and physicochemical properties of its water-soluble natural ingredients with their immunomodulatory activities and potential mechanisms. Firstly, we optimized the extraction method of glycoproteins from S. biaculeatus and prepared the crude glycoprotein SYB, from which the fraction glycoprotein SYB-1 was further purified. The carbohydrates of SYB-1 were 8.46% comprising mannose, glucose, and galactose, with an average molecular weight of 9.423 kDa. Amino acid analysis demonstrated that its major amino acids are glycine, glutamic acid, aspartic acid, and proline, with a total amino acid content of 88.81%. Furthermore, SYB-1 could significantly increase the cell viability of macrophages, and promote the release of NO, TNF-α, and IL-6. Metabolomics revealed that it was associated with arachidonic acid metabolism. The CYP450 enzyme family members and PTGS2 may be key targets for the regulatory role. These results suggested that the glycoprotein of S. biaculeatus may be an attractive functional food supplement from natural sources for immunocompromised populations.

Graphical Abstract

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双棘合藤糖蛋白的制备、理化性质及免疫调节活性研究。

双棘合藤是一种具有增强非特异性免疫功能的潜在海洋功能性食品，但其功能成分鲜有报道。因此，本研究重点对其水溶性天然成分的制备、理化性质、免疫调节活性及其潜在机制进行了研究。首先，优化双歧杆菌中糖蛋白的提取方法，制备粗糖蛋白SYB，并对粗糖蛋白SYB-1进行进一步纯化。SYB-1的碳水化合物含量为8.46%，由甘露糖、葡萄糖和半乳糖组成，平均分子量为9.423 kDa。氨基酸分析表明，其主要氨基酸为甘氨酸、谷氨酸、天冬氨酸和脯氨酸，总氨基酸含量为88.81%。SYB-1能显著提高巨噬细胞的细胞活力，促进NO、TNF-α、IL-6的释放。代谢组学结果显示，它与花生四烯酸代谢有关。CYP450酶家族成员和PTGS2可能是调控作用的关键靶点。这些结果表明，双歧杆菌的糖蛋白可能是免疫功能低下人群的一种有吸引力的天然功能性食品补充剂。

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来源期刊

Natural Products and Bioprospecting CHEMISTRY, MEDICINAL-

CiteScore

8.30

自引率

2.10%

发文量

审稿时长

13 weeks

期刊介绍： Natural Products and Bioprospecting serves as an international forum for essential research on natural products and focuses on, but is not limited to, the following aspects: Natural products: isolation and structure elucidation Natural products: synthesis Biological evaluation of biologically active natural products Bioorganic and medicinal chemistry Biosynthesis and microbiological transformation Fermentation and plant tissue cultures Bioprospecting of natural products from natural resources All research articles published in this journal have undergone rigorous peer review. In addition to original research articles, Natural Products and Bioprospecting publishes reviews and short communications, aiming to rapidly disseminate the research results of timely interest, and comprehensive reviews of emerging topics in all the areas of natural products. It is also an open access journal, which provides free access to its articles to anyone, anywhere.