Efficient extraction of Polygonum hydropiper L. Polyphenol using Dual-ligand Cu MOF and bioactive activity study of Polyphenol-MOF complex microparticles

Abstract

A novel dual-ligand Cu MOF material was synthesized with β-cyclodextrin and trimesic acid (H₃BTC) as the ligand of MOF and adsorbing material for plant polyphenol. The Box-Behnken design, a method used in response surface methodology, was applied to improve the extraction of Polygonum hydropiper L. (PL) polyphenols using the as-synthesized MOF. The optimal extraction conditions were determined to be a temperature of 39.4 °C, an ultrasound duration of 16.6 min, and a β-CD solution concentration of 13.4 mM. The resulting extract demonstrated a TPC of 196.34 µg GAE/mL, a DPPH radical scavenging activity of 75.33 µg GAE/mL, a FRAP activity of 68.53 µg GAE/mL, and an ABTS radical scavenging activity of 85.53 µg GAE/mL. The anti-glycosylation experiments demonstrated that the inclusion complex formed by PL polyphenols and β-CD effectively inhibits the production of end products during the later stages of glycosylation, suggesting that PL polyphenols possess strong anti-glycosylation properties. The bioactive activity study show that the copper-based MOFs (Cu-MOFs) possess good antitumor activity which leverages the unique characteristics of Fenton reactions and glutathione depletion capacity of Cu-MOFs. Furthermore, hemolysis test results demonstrated that the as-synthesized MOF possesses excellent biocompatibility, providing important evidence for its potential use in biomedical applications.

Graphical abstract

Schematic representation of β-cyclodextrin-assisted polyphenol extraction from Polygonum hydropiper L. and preparation of ketone-containing metal-organic frameworks.