Polymorphisms in TLR4 and IL6 and their association with obesity and insulin resistance in a Venezuelan population

IF 1.9 4区医学 Q4 ENDOCRINOLOGY & METABOLISM

Endocrinologia Diabetes Y Nutricion Pub Date : 2026-01-01 Epub Date: 2025-11-27 DOI:10.1016/j.endien.2025.501657

Stefani Hernández Osorio , María Fátima Garcés , Eva Salazar Alcalá , José Manuel Pestana , Mercedes Fernández Mestre

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Abstract

Objective

This study investigated the association between genetic polymorphisms in the TLR4 (rs4986790 and rs4986791) and IL6 (rs1800795) genes with obesity in a Venezuelan population.

Methods

We conducted a retrospective case-control study with 207 unrelated Venezuelans (137 obese, 70 normal weight). Blood samples were obtained to measure glucose, cholesterol, triglycerides, HDL, insulin, and insulin resistance (HOMA-IR ≥ 2.6) and genotype the TLR4 (rs4986790 and rs4986791) and IL6 (rs1800795) polymorphisms using PCR-SSP. Statistical tests: Student's t-test, Pearson correlation coefficient, chi-square test, odds ratio (OR) with 95%CI were used.

Results

The relationship between obesity and insulin resistance (IR) was confirmed. Heterozygous genotypes A/G (TLR4 rs4986790, P = .012) and C/T (TLR4 rs4986791, P = .005) were more frequent in normal-weight individuals, while homozygous genotypes A/A (TLR4 rs4986790, P = .012) and C/C (TLR4 rs4986791, P = .005) were more frequent in obese individuals. The C allele and CC genotype of IL6 rs1800795 (P = .007) were associated with obesity. The IL6 C/C genotype was associated with higher insulin and HOMA in obese individuals. In obese individuals with IR, the CC genotype (TLR4 rs4986791, P = .03) was more common, as opposted to CT, which was less common (P = .03). The C/C genotype of IL6 rs1800795 was more frequent in obese individuals with IR (P < .0001). Obese individuals with IR and the AA genotype of TLR4 rs4986790 had higher BMI, HOMA-IR, and glucose.

Conclusion

These findings suggest that genetic variations in the TLR4 and IL6 genes may predispose to obesity and its metabolic complications in the Venezuelan population.

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委内瑞拉人群中TLR4和IL6多态性及其与肥胖和胰岛素抵抗的关系

目的：本研究探讨了委内瑞拉人群中TLR4 （rs4986790和rs4986791）和IL6 （rs1800795）基因多态性与肥胖的关系。方法：我们对207名委内瑞拉人（137名肥胖，70名正常体重）进行了回顾性病例对照研究。采集血样，测定血糖、胆固醇、甘油三酯、HDL、胰岛素和胰岛素抵抗（HOMA-IR≥2.6），并利用PCR-SSP对TLR4 （rs4986790和rs4986791）和IL6 （rs1800795）多态性进行基因分型。统计学检验：采用学生t检验、Pearson相关系数、卡方检验、比值比（OR）， 95%CI。结果：肥胖与胰岛素抵抗（IR）的关系得到证实。杂合子基因型A/G (TLR4 rs4986790, P =；012)和C/T (TLR4 rs4986791, P =。而纯合子基因型A/A (TLR4 rs4986790, P =。012)和C/C (TLR4 rs4986791, P =。2005)在肥胖人群中更为常见。IL6 rs1800795的C等位基因和CC基因型与肥胖相关（P = 0.007）。在肥胖个体中，IL6 C/C基因型与较高的胰岛素和HOMA相关。在肥胖IR患者中，CC基因型(TLR4 rs4986791, P =。03)较常见，而CT较不常见（P = .03）。il - 6 rs1800795的C/C基因型在肥胖IR患者中更为常见(P结论：这些发现表明，TLR4和il - 6基因的遗传变异可能易导致委内瑞拉人群的肥胖及其代谢并发症。

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来源期刊

Endocrinologia Diabetes Y Nutricion Multiple-

CiteScore

2.10

自引率

10.50%

发文量

期刊介绍： Endocrinología, Diabetes y Nutrición is the official journal of the Spanish Society of Endocrinology and Nutrition (Sociedad Española de Endocrinología y Nutrición, SEEN) and the Spanish Society of Diabetes (Sociedad Española de Diabetes, SED), and was founded in 1954. The aim of the journal is to improve knowledge and be a useful tool in practice for clinical and laboratory specialists, trainee physicians, researchers, and nurses interested in endocrinology, diabetes, nutrition and related disciplines. It is an international journal published in Spanish (print and online) and English (online), covering different fields of endocrinology and metabolism, including diabetes, obesity, and nutrition disorders, as well as the most relevant research produced mainly in Spanish language territories. The quality of the contents is ensured by a prestigious national and international board, and by a selected panel of specialists involved in a rigorous peer review. The result is that only manuscripts containing high quality research and with utmost interest for clinicians and professionals related in the field are published. The Journal publishes Original clinical and research articles, Reviews, Special articles, Clinical Guidelines, Position Statements from both societies and Letters to the editor. Endocrinología, Diabetes y Nutrición can be found at Science Citation Index Expanded, Medline/PubMed and SCOPUS.